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• W2736706627 abstract "Organotypic in vitro culture of 3D spheroids in an extracellular matrix represent a promising cancer therapy prediction model for personalized medicine screens due to their controlled experimental conditions and physiological similarities to in vivo conditions. As in tumors in vivo, 3D invasion cultures identify intratumor heterogeneity of growth, invasion and apoptosis induction by cytotoxic therapy. We here combine in vitro 3D spheroid invasion culture with irradiation and automated nucleus-based segmentation for single cell analysis to quantify growth, survival, apoptosis and invasion response during experimental radiation therapy. As output, multi-parameter histogram-based representations deliver an integrated insight into therapy response and resistance. This workflow may be suited for high-throughput screening and identification of invasive and therapy-resistant tumor sub-populations." @default.
• W2736706627 created "2017-07-31" @default.
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• W2736706627 date "2017-09-01" @default.
• W2736706627 modified "2023-09-29" @default.
• W2736706627 title "Single cell-based automated quantification of therapy responses of invasive cancer spheroids in organotypic 3D culture" @default.
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• W2736706627 doi "https://doi.org/10.1016/j.ymeth.2017.07.015" @default.
• W2736706627 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28739118" @default.
• W2736706627 hasPublicationYear "2017" @default.
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