FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2783669395> ?p ?o ?g. }

• W2783669395 endingPage "130" @default.
• W2783669395 startingPage "124" @default.
• W2783669395 abstract "Reduced infectivity of phage due to the nebulization process has been reported previously, but no visual evidence on structural change upon nebulization has been established, or whether these structural changes can be attributed to the titer reduction. In this study, transmission electron microscopy (TEM) was employed to assess the impact of three different types of nebulizers, air-jet, vibrating-mesh and static-mesh nebulizers, on the structural stability of a Myoviridae phage, PEV44, active against Pseudomonas aeruginosa. The morphology of the phage in the nebulized samples was categorized into two groups: “whole” (the capsid and tail held together) and “broken” (the capsid separated from the tail) phages. The “whole” phage group was further divided into three sub-groups: (1) intact, (2) contracted tail and (3) empty capsid. The starting stock suspension was found to contain considerable portions of “broken” phages (35.5 ± 6.7%), “whole” phages with contracted tails (9.9 ± 5.4%) and empty capsids (19.3 ± 8.9%). The fraction of “broken” phages was significantly increased after nebulization, with the air-jet nebulizer (83%) being more pronounced than the mesh type nebulizers (50–60%). While the amount of intact phages (2–10%) and whole phages with contracted tails (0–3%) were significantly decreased, the proportion of phages with empty capsids (15–36%) were not significantly different. Phages with broken features obtained by TEM quantification were 92.9 ± 3.2%, 74.8 ± 10.4% and 71.2 ± 11.0% for the jet, vibrating-mesh and static-mesh nebulizers, respectively. These results were found to be comparable with the titer loss obtained by the conventional plaque assay results. The in vitro aerosol performance and viable phage delivery of the three nebulizers was also assessed. The Omron nebulizer achieved a significantly higher viable respirable fraction (VRF) than the SideStream and Aeroneb Go (15.1 ± 5.8%, 2.4 ± 2.0%, 4.1 ± 2.7% respectively). In conclusion, this study identified various changes in the phage structure and viability of phage from different types of nebulizers. Understanding these effects and the phage tolerance to nebulization stresses can potentially improve our choice of the delivery method for inhaled phage therapy." @default.
• W2783669395 created "2018-01-26" @default.
• W2783669395 creator A5025667640 @default.
• W2783669395 creator A5048069105 @default.
• W2783669395 creator A5050840890 @default.
• W2783669395 creator A5056076266 @default.
• W2783669395 creator A5067686421 @default.
• W2783669395 date "2018-04-01" @default.
• W2783669395 modified "2023-10-01" @default.
• W2783669395 title "Nebulization effects on structural stability of bacteriophage PEV 44" @default.
• W2783669395 cites W1480966304 @default.
• W2783669395 cites W1960529837 @default.
• W2783669395 cites W1972740035 @default.
• W2783669395 cites W1978538873 @default.
• W2783669395 cites W1981962112 @default.
• W2783669395 cites W1989131500 @default.
• W2783669395 cites W1991772704 @default.
• W2783669395 cites W2004106896 @default.
• W2783669395 cites W2021453492 @default.
• W2783669395 cites W2040515264 @default.
• W2783669395 cites W2042616926 @default.
• W2783669395 cites W2067626761 @default.
• W2783669395 cites W2075138421 @default.
• W2783669395 cites W2076706379 @default.
• W2783669395 cites W2090817404 @default.
• W2783669395 cites W2107809565 @default.
• W2783669395 cites W2126711246 @default.
• W2783669395 cites W2130418188 @default.
• W2783669395 cites W2147622170 @default.
• W2783669395 cites W2150812319 @default.
• W2783669395 cites W2153421727 @default.
• W2783669395 cites W2159819650 @default.
• W2783669395 cites W2345835617 @default.
• W2783669395 cites W2498162173 @default.
• W2783669395 cites W2524418100 @default.
• W2783669395 cites W2670200616 @default.
• W2783669395 doi "https://doi.org/10.1016/j.ejpb.2018.01.010" @default.
• W2783669395 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29353018" @default.
• W2783669395 hasPublicationYear "2018" @default.
• W2783669395 type Work @default.
• W2783669395 sameAs 2783669395 @default.
• W2783669395 citedByCount "34" @default.
• W2783669395 countsByYear W27836693952018 @default.
• W2783669395 countsByYear W27836693952019 @default.
• W2783669395 countsByYear W27836693952020 @default.
• W2783669395 countsByYear W27836693952021 @default.
• W2783669395 countsByYear W27836693952022 @default.
• W2783669395 countsByYear W27836693952023 @default.
• W2783669395 crossrefType "journal-article" @default.
• W2783669395 hasAuthorship W2783669395A5025667640 @default.
• W2783669395 hasAuthorship W2783669395A5048069105 @default.
• W2783669395 hasAuthorship W2783669395A5050840890 @default.
• W2783669395 hasAuthorship W2783669395A5056076266 @default.
• W2783669395 hasAuthorship W2783669395A5067686421 @default.
• W2783669395 hasBestOaLocation W27836693952 @default.
• W2783669395 hasConcept C104317684 @default.
• W2783669395 hasConcept C120665830 @default.
• W2783669395 hasConcept C121332964 @default.
• W2783669395 hasConcept C12554922 @default.
• W2783669395 hasConcept C166029249 @default.
• W2783669395 hasConcept C185592680 @default.
• W2783669395 hasConcept C202878990 @default.
• W2783669395 hasConcept C2776441376 @default.
• W2783669395 hasConcept C2780352487 @default.
• W2783669395 hasConcept C42219234 @default.
• W2783669395 hasConcept C547475151 @default.
• W2783669395 hasConcept C55493867 @default.
• W2783669395 hasConcept C71924100 @default.
• W2783669395 hasConcept C86803240 @default.
• W2783669395 hasConcept C93877712 @default.
• W2783669395 hasConceptScore W2783669395C104317684 @default.
• W2783669395 hasConceptScore W2783669395C120665830 @default.
• W2783669395 hasConceptScore W2783669395C121332964 @default.
• W2783669395 hasConceptScore W2783669395C12554922 @default.
• W2783669395 hasConceptScore W2783669395C166029249 @default.
• W2783669395 hasConceptScore W2783669395C185592680 @default.
• W2783669395 hasConceptScore W2783669395C202878990 @default.
• W2783669395 hasConceptScore W2783669395C2776441376 @default.
• W2783669395 hasConceptScore W2783669395C2780352487 @default.
• W2783669395 hasConceptScore W2783669395C42219234 @default.
• W2783669395 hasConceptScore W2783669395C547475151 @default.
• W2783669395 hasConceptScore W2783669395C55493867 @default.
• W2783669395 hasConceptScore W2783669395C71924100 @default.
• W2783669395 hasConceptScore W2783669395C86803240 @default.
• W2783669395 hasConceptScore W2783669395C93877712 @default.
• W2783669395 hasFunder F4320334704 @default.
• W2783669395 hasLocation W27836693951 @default.
• W2783669395 hasLocation W27836693952 @default.
• W2783669395 hasLocation W27836693953 @default.
• W2783669395 hasOpenAccess W2783669395 @default.
• W2783669395 hasPrimaryLocation W27836693951 @default.
• W2783669395 hasRelatedWork W1992536307 @default.
• W2783669395 hasRelatedWork W2000073780 @default.
• W2783669395 hasRelatedWork W2022664742 @default.
• W2783669395 hasRelatedWork W2073912448 @default.
• W2783669395 hasRelatedWork W2086856705 @default.
• W2783669395 hasRelatedWork W2101895029 @default.
• W2783669395 hasRelatedWork W2146987229 @default.

• next