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• W2883282783 abstract "An in vitro multienzyme synthetic system was developed and optimized to efficiently produce kaempferol in a single reaction tube. Two key genes, Atf3h and Atfls1, in the biosynthetic pathway of kaempferol were cloned into a prokaryotic expression vector and overexpressed in Escherichia coli. The recombinant proteins were purified through affinity chromatography and showed activities of flavanone 3-hydroxylase and flavonol synthase, respectively, followed by development of an in vitro synthetic system for producing kaempferol. The system contains 8.2 mM α-ketoglutaric acid, 0.01 mM ferrous ion, 0.4% sodium ascorbate, 25 μg/mL of each recombinant enzyme, and 10% glycerol in 100 mM Tris-HCl (pH 7.2). When the reaction was carried out at 40 °C for 40–50 min, the yield of kaempferol was 37.55 ± 1.62 mg/L and the conversion rate from NRN to KMF was 55.89% ± 2.74%. Overall, this system provides a promising and efficient approach to produce kaempferol economically." @default.
• W2883282783 created "2018-08-03" @default.
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• W2883282783 date "2018-07-18" @default.
• W2883282783 modified "2023-10-17" @default.
• W2883282783 title "Development and Optimization of an In Vitro Multienzyme Synthetic System for Production of Kaempferol from Naringenin" @default.
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• W2883282783 doi "https://doi.org/10.1021/acs.jafc.8b01299" @default.
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