FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2884378529> ?p ?o ?g. }

• W2884378529 endingPage "9321" @default.
• W2884378529 startingPage "9315" @default.
• W2884378529 abstract "Single-molecule fluorescence imaging is a promising strategy for biomolecule detection. However, the accuracy of single-molecule method is often compromised by the false-positive events at the ultralow sample levels that are caused by the nonspecific adsorption of the fluorescent labeled probe and other fluorescent impurities on the imaging surface. Here, we demonstrate an ultrasensitive single molecule detection assay based on dual-color fluorescent colocalization of spilt aptamers that was implemented to the measurement of adenosine triphosphate (ATP). The ATP aptamer was split into two fragments and labeled with green and red dye molecules, respectively. When the two probes of split aptamers were brought together by the target ATP molecule, the two colors of fluorescence of two probes were simultaneously detected through two channels and projected to the correlated locations in the two halves of image. The colocalizaiton imaging of two split apatamer probes greatly excluded the false detection of biomolecules that was usually caused by the fluorescent noise of single nonbound aptamer probes and impurities, and further improved the accuracy of measurement. The assay showed excellent selectivity and high sensitivity for ATP detection with linear range of 1 pM to 5 nM and a detection limit of 100 fM. This versatile protocol of single molecule colocalization of split apatamer can be widely applied to the ultrasensitive and highly accurate detection of many types of biomolecules in basic research and biomedical applications." @default.
• W2884378529 created "2018-08-03" @default.
• W2884378529 creator A5012926962 @default.
• W2884378529 creator A5016038041 @default.
• W2884378529 creator A5060480074 @default.
• W2884378529 creator A5066586426 @default.
• W2884378529 creator A5073694314 @default.
• W2884378529 creator A5086060866 @default.
• W2884378529 date "2018-07-13" @default.
• W2884378529 modified "2023-10-10" @default.
• W2884378529 title "Single Molecule Fluorescent Colocalization of Split Aptamers for Ultrasensitive Detection of Biomolecules" @default.
• W2884378529 cites W1589374402 @default.
• W2884378529 cites W1972046892 @default.
• W2884378529 cites W1984954149 @default.
• W2884378529 cites W2000079619 @default.
• W2884378529 cites W2002120250 @default.
• W2884378529 cites W2004044200 @default.
• W2884378529 cites W2025258376 @default.
• W2884378529 cites W2027350203 @default.
• W2884378529 cites W2045641607 @default.
• W2884378529 cites W2052826004 @default.
• W2884378529 cites W2061909211 @default.
• W2884378529 cites W2070296029 @default.
• W2884378529 cites W2070363388 @default.
• W2884378529 cites W2074184480 @default.
• W2884378529 cites W2075768046 @default.
• W2884378529 cites W2076034830 @default.
• W2884378529 cites W2077151519 @default.
• W2884378529 cites W2078505562 @default.
• W2884378529 cites W2081627119 @default.
• W2884378529 cites W2121236750 @default.
• W2884378529 cites W2128423073 @default.
• W2884378529 cites W2133019322 @default.
• W2884378529 cites W2156063722 @default.
• W2884378529 cites W2168509058 @default.
• W2884378529 cites W2174314186 @default.
• W2884378529 cites W2314221437 @default.
• W2884378529 cites W2316501172 @default.
• W2884378529 cites W2332894578 @default.
• W2884378529 cites W2334865985 @default.
• W2884378529 cites W2342863872 @default.
• W2884378529 cites W2344450955 @default.
• W2884378529 cites W2396762320 @default.
• W2884378529 cites W2400739184 @default.
• W2884378529 cites W2473515804 @default.
• W2884378529 cites W2494227803 @default.
• W2884378529 cites W2517162516 @default.
• W2884378529 cites W2581026338 @default.
• W2884378529 cites W2589310748 @default.
• W2884378529 cites W2594402484 @default.
• W2884378529 cites W2624001534 @default.
• W2884378529 cites W2743588870 @default.
• W2884378529 cites W2757348710 @default.
• W2884378529 cites W2767681889 @default.
• W2884378529 cites W2788985185 @default.
• W2884378529 cites W2790185121 @default.
• W2884378529 doi "https://doi.org/10.1021/acs.analchem.8b01916" @default.
• W2884378529 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30003776" @default.
• W2884378529 hasPublicationYear "2018" @default.
• W2884378529 type Work @default.
• W2884378529 sameAs 2884378529 @default.
• W2884378529 citedByCount "32" @default.
• W2884378529 countsByYear W28843785292019 @default.
• W2884378529 countsByYear W28843785292020 @default.
• W2884378529 countsByYear W28843785292021 @default.
• W2884378529 countsByYear W28843785292022 @default.
• W2884378529 countsByYear W28843785292023 @default.
• W2884378529 crossrefType "journal-article" @default.
• W2884378529 hasAuthorship W2884378529A5012926962 @default.
• W2884378529 hasAuthorship W2884378529A5016038041 @default.
• W2884378529 hasAuthorship W2884378529A5060480074 @default.
• W2884378529 hasAuthorship W2884378529A5066586426 @default.
• W2884378529 hasAuthorship W2884378529A5073694314 @default.
• W2884378529 hasAuthorship W2884378529A5086060866 @default.
• W2884378529 hasConcept C10390740 @default.
• W2884378529 hasConcept C119128265 @default.
• W2884378529 hasConcept C120665830 @default.
• W2884378529 hasConcept C121332964 @default.
• W2884378529 hasConcept C12554922 @default.
• W2884378529 hasConcept C128972844 @default.
• W2884378529 hasConcept C153911025 @default.
• W2884378529 hasConcept C178790620 @default.
• W2884378529 hasConcept C185592680 @default.
• W2884378529 hasConcept C32909587 @default.
• W2884378529 hasConcept C40209533 @default.
• W2884378529 hasConcept C43617362 @default.
• W2884378529 hasConcept C49853544 @default.
• W2884378529 hasConcept C55493867 @default.
• W2884378529 hasConcept C86803240 @default.
• W2884378529 hasConcept C91881484 @default.
• W2884378529 hasConceptScore W2884378529C10390740 @default.
• W2884378529 hasConceptScore W2884378529C119128265 @default.
• W2884378529 hasConceptScore W2884378529C120665830 @default.
• W2884378529 hasConceptScore W2884378529C121332964 @default.
• W2884378529 hasConceptScore W2884378529C12554922 @default.
• W2884378529 hasConceptScore W2884378529C128972844 @default.
• W2884378529 hasConceptScore W2884378529C153911025 @default.
• W2884378529 hasConceptScore W2884378529C178790620 @default.

• next