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• W2890432499 abstract "Magnetic resonance imaging (MRI)-guided pulsed focused ultrasound (pFUS) combined with microbubbles (MB) contrast agent infusion has been shown to transiently disrupt the blood-brain barrier (BBBD), increasing the delivery of neurotherapeutics to treat central nervous system (CNS) diseases. pFUS interaction with the intravascular MB results in acoustic cavitation forces passing through the neurovascular unit (NVU), inducing BBBD detected on contrast-enhanced MRI. Multiple pFUS+MB exposures in Alzheimer's disease (AD) models are being investigated as a method to clear amyloid plaques by activated microglia or infiltrating immune cells. Since it has been reported that pFUS+MB can induce a sterile inflammatory response (SIR) [1-5] in the rat, the goal of this study was to investigate the potential long-term effects of SIR in the brain following single and six weekly sonications by serial high-resolution MRI and pathology. Methods: Female Sprague Dawley rats weighing 217±16.6 g prior to sonication received bromo-deoxyuridine (BrdU) to tag proliferating cells in the brain. pFUS was performed at 548 kHz, ultrasound burst 10 ms and initial peak negative pressure of 0.3 MPa (in water) for 120 s coupled with a slow infusion of ~460 µL/kg (5-8×107 MB) that started 30 s before and 30 s during sonication. Nine 2 mm focal regions in the left cortex and four regions over the right hippocampus were treated with pFUS+MB. Serial high-resolution brain MRIs at 3 T and 9.4 T were obtained following a single or during the course of six weekly pFUS+MB resulting in BBBD in the left cortex and the right hippocampus. Animals were monitored over 7 to 13 weeks and imaging results were compared to histology. Results: Fewer than half of the rats receiving a single pFUS+MB exposure displayed hypointense voxels on T2*-weighted (w) MRI at week 7 or 13 in the cortex or hippocampus without differences compared to the contralateral side on histograms of T2* maps. Single sonicated rats had evidence of limited microglia activation on pathology compared to the contralateral hemisphere. Six weekly pFUS+MB treatments resulted in pathological changes on T2*w images with multiple hypointense regions, cortical atrophy, along with 50% of rats having persistent BBBD and astrogliosis by MRI. Pathologic analysis of the multiple sonicated animals demonstrated the presence of metallophagocytic Prussian blue-positive cells in the parenchyma with significantly (p<0.05) increased areas of activated astrocytes and microglia, and high numbers of systemic infiltrating CD68+ macrophages along with BrdU+ cells compared to contralateral brain. In addition, multiple treatments caused an increase in the number of hyperphosphorylated Tau (pTau)-positive neurons containing neurofibrillary tangles (NFT) in the sonicated cortex but not in the hippocampus when compared to contralateral brain, which was confirmed by Western blot (WB) (p<0.04). Conclusions: The repeated SIR following multiple pFUS+MB treatments could contribute to changes on MR imaging including persistent BBBD, cortical atrophy, and hypointense voxels on T2w and T2*w images consistent with pathological injury. Moreover, areas of astrogliosis, activated microglia, along with higher numbers of CD68+ infiltrating macrophages and BrdU+ cells were detected in multiple sonicated areas of the cortex and hippocampus. Elevations in pTau and NFT were detected in neurons of the multiple sonicated cortex. Minimal changes on MRI and histology were observed in single pFUS+MB-treated rats at 7 and 13 weeks post sonication. In comparison, animals that received 6 weekly sonications demonstrated evidence on MRI and histology of vascular damage, inflammation and neurodegeneration associated with the NVU commonly observed in trauma. Further investigation is recommended of the long-term effects of multiple pFUS+MB in clinical trials." @default.
• W2890432499 created "2018-09-27" @default.
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• W2890432499 date "2018-01-01" @default.
• W2890432499 modified "2023-09-28" @default.
• W2890432499 title "MRI and histological evaluation of pulsed focused ultrasound and microbubbles treatment effects in the brain" @default.
• W2890432499 cites W1151137276 @default.
• W2890432499 cites W1515328725 @default.
• W2890432499 cites W1585793628 @default.
• W2890432499 cites W1588685142 @default.
• W2890432499 cites W1608225731 @default.
• W2890432499 cites W1608593799 @default.
• W2890432499 cites W1801991441 @default.
• W2890432499 cites W1855337837 @default.
• W2890432499 cites W1874972851 @default.
• W2890432499 cites W1953231210 @default.
• W2890432499 cites W1964956174 @default.
• W2890432499 cites W1968052832 @default.
• W2890432499 cites W1972683821 @default.
• W2890432499 cites W1982760076 @default.
• W2890432499 cites W1986371611 @default.
• W2890432499 cites W1997709907 @default.
• W2890432499 cites W2000663601 @default.
• W2890432499 cites W2002543308 @default.
• W2890432499 cites W2011634611 @default.
• W2890432499 cites W2014357012 @default.
• W2890432499 cites W2015026123 @default.
• W2890432499 cites W2017589879 @default.
• W2890432499 cites W2017984925 @default.
• W2890432499 cites W2018522439 @default.
• W2890432499 cites W2020531776 @default.
• W2890432499 cites W2021502355 @default.
• W2890432499 cites W2022047142 @default.
• W2890432499 cites W2025817925 @default.
• W2890432499 cites W2035920357 @default.
• W2890432499 cites W2036426873 @default.
• W2890432499 cites W2037934563 @default.
• W2890432499 cites W2040498125 @default.
• W2890432499 cites W2041413252 @default.
• W2890432499 cites W2042851093 @default.
• W2890432499 cites W2043896159 @default.
• W2890432499 cites W2045869644 @default.
• W2890432499 cites W2046245241 @default.
• W2890432499 cites W2050938473 @default.
• W2890432499 cites W2052864664 @default.
• W2890432499 cites W2053068006 @default.
• W2890432499 cites W2054714985 @default.
• W2890432499 cites W2055300560 @default.
• W2890432499 cites W2055797258 @default.
• W2890432499 cites W2068597696 @default.
• W2890432499 cites W2071956340 @default.
• W2890432499 cites W2071996546 @default.
• W2890432499 cites W2072835186 @default.
• W2890432499 cites W2073077090 @default.
• W2890432499 cites W2081325915 @default.
• W2890432499 cites W2090175192 @default.
• W2890432499 cites W2091184929 @default.
• W2890432499 cites W2093980521 @default.
• W2890432499 cites W2094299440 @default.
• W2890432499 cites W2097971052 @default.
• W2890432499 cites W2098741244 @default.
• W2890432499 cites W2100559373 @default.
• W2890432499 cites W2101504183 @default.
• W2890432499 cites W2103825400 @default.
• W2890432499 cites W2108277337 @default.
• W2890432499 cites W2114072420 @default.
• W2890432499 cites W2114596635 @default.
• W2890432499 cites W2115362657 @default.
• W2890432499 cites W2116063788 @default.
• W2890432499 cites W2116784956 @default.
• W2890432499 cites W2118694152 @default.
• W2890432499 cites W2118879132 @default.
• W2890432499 cites W2122021547 @default.
• W2890432499 cites W2126680127 @default.
• W2890432499 cites W2128610705 @default.
• W2890432499 cites W2129941547 @default.
• W2890432499 cites W2131358222 @default.
• W2890432499 cites W2135179728 @default.
• W2890432499 cites W2135188102 @default.
• W2890432499 cites W2135516392 @default.
• W2890432499 cites W2135637880 @default.
• W2890432499 cites W2151467737 @default.
• W2890432499 cites W2154096369 @default.
• W2890432499 cites W2161323379 @default.
• W2890432499 cites W2166980283 @default.
• W2890432499 cites W2169076271 @default.
• W2890432499 cites W2171296382 @default.
• W2890432499 cites W2172864295 @default.
• W2890432499 cites W2180223963 @default.
• W2890432499 cites W2185034389 @default.
• W2890432499 cites W2189621653 @default.

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