FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2893886210> ?p ?o ?g. }

• W2893886210 endingPage "e0204487" @default.
• W2893886210 startingPage "e0204487" @default.
• W2893886210 abstract "We used the CRISPR/Cas9 system to knock-in reporter transgenes at the kidney injury molecule-1 (KIM-1) locus and isolated human proximal tubule cell (HK-2) clones. PCR verified targeted knock-in of the luciferase and eGFP reporter at the KIM-1 locus. HK-2-KIM-1 reporter cells responded to various stimuli including hypoxia, cisplatin, and high glucose, indicative of upregulation of KIM-1 expression. We attempted using CRISPR/Cas9 to also engineer the KIM-1 reporter in telomerase-immortalized human RPTEC cells. However, these cells demonstrated an inability to undergo homologous recombination at the target locus. KIM-1-reporter human proximal tubular cells could be valuable tools in drug discovery for molecules inhibiting kidney injury. Additionally, our gene targeting strategy could be used in other cell lines to evaluate the biology of KIM-1 in vitro or in vivo." @default.
• W2893886210 created "2018-10-05" @default.
• W2893886210 creator A5052684400 @default.
• W2893886210 creator A5073578645 @default.
• W2893886210 date "2018-09-27" @default.
• W2893886210 modified "2023-10-15" @default.
• W2893886210 title "CRISPR/Cas9 engineering of a KIM-1 reporter human proximal tubule cell line" @default.
• W2893886210 cites W1988838635 @default.
• W2893886210 cites W1996788258 @default.
• W2893886210 cites W2036176224 @default.
• W2893886210 cites W2041929136 @default.
• W2893886210 cites W2045421375 @default.
• W2893886210 cites W2064815984 @default.
• W2893886210 cites W2068446361 @default.
• W2893886210 cites W2104305817 @default.
• W2893886210 cites W2122563582 @default.
• W2893886210 cites W2131419242 @default.
• W2893886210 cites W2162159731 @default.
• W2893886210 cites W2164819474 @default.
• W2893886210 cites W2164995160 @default.
• W2893886210 cites W2171113167 @default.
• W2893886210 cites W2195071417 @default.
• W2893886210 cites W2440599929 @default.
• W2893886210 cites W2469359910 @default.
• W2893886210 cites W2517401203 @default.
• W2893886210 cites W2564377385 @default.
• W2893886210 doi "https://doi.org/10.1371/journal.pone.0204487" @default.
• W2893886210 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6160069" @default.
• W2893886210 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30260998" @default.
• W2893886210 hasPublicationYear "2018" @default.
• W2893886210 type Work @default.
• W2893886210 sameAs 2893886210 @default.
• W2893886210 citedByCount "7" @default.
• W2893886210 countsByYear W28938862102019 @default.
• W2893886210 countsByYear W28938862102020 @default.
• W2893886210 countsByYear W28938862102021 @default.
• W2893886210 countsByYear W28938862102022 @default.
• W2893886210 crossrefType "journal-article" @default.
• W2893886210 hasAuthorship W2893886210A5052684400 @default.
• W2893886210 hasAuthorship W2893886210A5073578645 @default.
• W2893886210 hasBestOaLocation W28938862101 @default.
• W2893886210 hasConcept C102230213 @default.
• W2893886210 hasConcept C102744134 @default.
• W2893886210 hasConcept C104317684 @default.
• W2893886210 hasConcept C111829913 @default.
• W2893886210 hasConcept C132455925 @default.
• W2893886210 hasConcept C142613039 @default.
• W2893886210 hasConcept C150194340 @default.
• W2893886210 hasConcept C153911025 @default.
• W2893886210 hasConcept C161733203 @default.
• W2893886210 hasConcept C174510640 @default.
• W2893886210 hasConcept C2796436 @default.
• W2893886210 hasConcept C54009773 @default.
• W2893886210 hasConcept C54355233 @default.
• W2893886210 hasConcept C81885089 @default.
• W2893886210 hasConcept C86803240 @default.
• W2893886210 hasConcept C95444343 @default.
• W2893886210 hasConcept C98108389 @default.
• W2893886210 hasConceptScore W2893886210C102230213 @default.
• W2893886210 hasConceptScore W2893886210C102744134 @default.
• W2893886210 hasConceptScore W2893886210C104317684 @default.
• W2893886210 hasConceptScore W2893886210C111829913 @default.
• W2893886210 hasConceptScore W2893886210C132455925 @default.
• W2893886210 hasConceptScore W2893886210C142613039 @default.
• W2893886210 hasConceptScore W2893886210C150194340 @default.
• W2893886210 hasConceptScore W2893886210C153911025 @default.
• W2893886210 hasConceptScore W2893886210C161733203 @default.
• W2893886210 hasConceptScore W2893886210C174510640 @default.
• W2893886210 hasConceptScore W2893886210C2796436 @default.
• W2893886210 hasConceptScore W2893886210C54009773 @default.
• W2893886210 hasConceptScore W2893886210C54355233 @default.
• W2893886210 hasConceptScore W2893886210C81885089 @default.
• W2893886210 hasConceptScore W2893886210C86803240 @default.
• W2893886210 hasConceptScore W2893886210C95444343 @default.
• W2893886210 hasConceptScore W2893886210C98108389 @default.
• W2893886210 hasFunder F4320306127 @default.
• W2893886210 hasFunder F4320337357 @default.
• W2893886210 hasIssue "9" @default.
• W2893886210 hasLocation W28938862101 @default.
• W2893886210 hasLocation W28938862102 @default.
• W2893886210 hasLocation W28938862103 @default.
• W2893886210 hasLocation W28938862104 @default.
• W2893886210 hasOpenAccess W2893886210 @default.
• W2893886210 hasPrimaryLocation W28938862101 @default.
• W2893886210 hasRelatedWork W1495450087 @default.
• W2893886210 hasRelatedWork W2011881800 @default.
• W2893886210 hasRelatedWork W2026463378 @default.
• W2893886210 hasRelatedWork W2055543925 @default.
• W2893886210 hasRelatedWork W2074364455 @default.
• W2893886210 hasRelatedWork W2099803033 @default.
• W2893886210 hasRelatedWork W2279781393 @default.
• W2893886210 hasRelatedWork W3011209998 @default.
• W2893886210 hasRelatedWork W3023455565 @default.
• W2893886210 hasRelatedWork W3026098209 @default.
• W2893886210 hasVolume "13" @default.
• W2893886210 isParatext "false" @default.
• W2893886210 isRetracted "false" @default.
• W2893886210 magId "2893886210" @default.

• next