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• W2895920199 abstract "Due to the lack of germline transmitting pluripotent stem cells (PSCs) cell lines and the extreme difficulty of somatic cell nuclear transfer (SCNT) in rabbit, the gene targeting technology in rabbit was lagging far behind those in rodents and in farm animals. As a result, the development and application of genetically engineered rabbit model are much limited. With the advent of gene editing nucleases, including ZFN, TALEN, and CRISPR/Cas9, it is now possible to produce gene targeting (i.e., knockout, knockin) rabbits with high success rates. In this chapter, we describe a comprehensive, step-by-step protocol for rabbit genome editing based on gene editing nucleases with specific emphasis of CRISPR/Cas9." @default.
• W2895920199 created "2018-10-26" @default.
• W2895920199 creator A5007667712 @default.
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• W2895920199 date "2018-10-24" @default.
• W2895920199 modified "2023-10-16" @default.
• W2895920199 title "Generation of Rabbit Models by Gene Editing Nucleases" @default.
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• W2895920199 doi "https://doi.org/10.1007/978-1-4939-8831-0_19" @default.
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