Matches in SemOpenAlex for { <https://semopenalex.org/work/W2907029242> ?p ?o ?g. }
- W2907029242 endingPage "2432" @default.
- W2907029242 startingPage "2428" @default.
- W2907029242 abstract "Abstract This work reports the one‐pot enzymatic cascade that completely converts l ‐arabinose to l ‐ribulose using four reactions catalyzed by pyranose 2‐oxidase (P2O), xylose reductase, formate dehydrogenase, and catalase. As wild‐type P2O is specific for the oxidation of six‐carbon sugars, a pool of P2O variants was generated based on rational design to change the specificity of the enzyme towards the oxidation of l ‐arabinose at the C2‐position. The variant T169G was identified as the best candidate, and this had an approximately 40‐fold higher rate constant for the flavin reduction (sugar oxidation) step, as compared to the wild‐type enzyme. Computational calculations using quantum mechanics/molecular mechanics (QM/MM) molecular dynamics (MD) showed that this improvement is due to a decrease in the steric effects at the axial C4‐OH of l ‐arabinose, which allows a reduction in the distance between the C2‐H and flavin N5, facilitating hydride transfer and enabling flavin reduction." @default.
- W2907029242 created "2019-01-11" @default.
- W2907029242 creator A5010206194 @default.
- W2907029242 creator A5017369266 @default.
- W2907029242 creator A5022102309 @default.
- W2907029242 creator A5031181844 @default.
- W2907029242 creator A5031302900 @default.
- W2907029242 creator A5036937612 @default.
- W2907029242 creator A5055016425 @default.
- W2907029242 creator A5074973966 @default.
- W2907029242 creator A5081699843 @default.
- W2907029242 date "2019-01-29" @default.
- W2907029242 modified "2023-10-14" @default.
- W2907029242 title "One‐Pot Bioconversion of <scp>l</scp> ‐Arabinose to <scp>l</scp> ‐Ribulose in an Enzymatic Cascade" @default.
- W2907029242 cites W1666892285 @default.
- W2907029242 cites W1969767016 @default.
- W2907029242 cites W2004049267 @default.
- W2907029242 cites W2005095483 @default.
- W2907029242 cites W2015793616 @default.
- W2907029242 cites W2016353709 @default.
- W2907029242 cites W2033592467 @default.
- W2907029242 cites W2046920360 @default.
- W2907029242 cites W2062214229 @default.
- W2907029242 cites W2064225836 @default.
- W2907029242 cites W2067013726 @default.
- W2907029242 cites W2072431472 @default.
- W2907029242 cites W2083129709 @default.
- W2907029242 cites W2093009294 @default.
- W2907029242 cites W2097672278 @default.
- W2907029242 cites W2103896911 @default.
- W2907029242 cites W2110919001 @default.
- W2907029242 cites W2121197874 @default.
- W2907029242 cites W2155033125 @default.
- W2907029242 cites W2167340980 @default.
- W2907029242 cites W2169461081 @default.
- W2907029242 cites W2328315067 @default.
- W2907029242 cites W2785293120 @default.
- W2907029242 cites W2887676429 @default.
- W2907029242 cites W2951303907 @default.
- W2907029242 cites W4251149349 @default.
- W2907029242 cites W2008968080 @default.
- W2907029242 doi "https://doi.org/10.1002/anie.201814219" @default.
- W2907029242 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30605256" @default.
- W2907029242 hasPublicationYear "2019" @default.
- W2907029242 type Work @default.
- W2907029242 sameAs 2907029242 @default.
- W2907029242 citedByCount "27" @default.
- W2907029242 countsByYear W29070292422019 @default.
- W2907029242 countsByYear W29070292422020 @default.
- W2907029242 countsByYear W29070292422021 @default.
- W2907029242 countsByYear W29070292422022 @default.
- W2907029242 countsByYear W29070292422023 @default.
- W2907029242 crossrefType "journal-article" @default.
- W2907029242 hasAuthorship W2907029242A5010206194 @default.
- W2907029242 hasAuthorship W2907029242A5017369266 @default.
- W2907029242 hasAuthorship W2907029242A5022102309 @default.
- W2907029242 hasAuthorship W2907029242A5031181844 @default.
- W2907029242 hasAuthorship W2907029242A5031302900 @default.
- W2907029242 hasAuthorship W2907029242A5036937612 @default.
- W2907029242 hasAuthorship W2907029242A5055016425 @default.
- W2907029242 hasAuthorship W2907029242A5074973966 @default.
- W2907029242 hasAuthorship W2907029242A5081699843 @default.
- W2907029242 hasConcept C100544194 @default.
- W2907029242 hasConcept C181199279 @default.
- W2907029242 hasConcept C185592680 @default.
- W2907029242 hasConcept C2776084176 @default.
- W2907029242 hasConcept C2779966244 @default.
- W2907029242 hasConcept C2780294150 @default.
- W2907029242 hasConcept C33093398 @default.
- W2907029242 hasConcept C34490408 @default.
- W2907029242 hasConcept C50212798 @default.
- W2907029242 hasConcept C55493867 @default.
- W2907029242 hasConcept C71240020 @default.
- W2907029242 hasConceptScore W2907029242C100544194 @default.
- W2907029242 hasConceptScore W2907029242C181199279 @default.
- W2907029242 hasConceptScore W2907029242C185592680 @default.
- W2907029242 hasConceptScore W2907029242C2776084176 @default.
- W2907029242 hasConceptScore W2907029242C2779966244 @default.
- W2907029242 hasConceptScore W2907029242C2780294150 @default.
- W2907029242 hasConceptScore W2907029242C33093398 @default.
- W2907029242 hasConceptScore W2907029242C34490408 @default.
- W2907029242 hasConceptScore W2907029242C50212798 @default.
- W2907029242 hasConceptScore W2907029242C55493867 @default.
- W2907029242 hasConceptScore W2907029242C71240020 @default.
- W2907029242 hasFunder F4320322614 @default.
- W2907029242 hasIssue "8" @default.
- W2907029242 hasLocation W29070292421 @default.
- W2907029242 hasLocation W29070292422 @default.
- W2907029242 hasOpenAccess W2907029242 @default.
- W2907029242 hasPrimaryLocation W29070292421 @default.
- W2907029242 hasRelatedWork W1967086378 @default.
- W2907029242 hasRelatedWork W1968592917 @default.
- W2907029242 hasRelatedWork W1973248560 @default.
- W2907029242 hasRelatedWork W2007764485 @default.
- W2907029242 hasRelatedWork W2018662142 @default.
- W2907029242 hasRelatedWork W2084592217 @default.
- W2907029242 hasRelatedWork W2102469838 @default.
- W2907029242 hasRelatedWork W2159055716 @default.