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• W2912175037 endingPage "3567" @default.
• W2912175037 startingPage "3550" @default.
• W2912175037 abstract "Activation of the checkpoint protein Tel1 requires the Mre11–Rad50–Xrs2 (MRX) complex, which recruits Tel1 at DNA double-strand breaks (DSBs) through direct interaction between Tel1 and Xrs2. However, in vitro Tel1 activation by MRX requires ATP binding to Rad50, suggesting a role also for the MR subcomplex in Tel1 activation. Here we describe two separation-of-functions alleles, mre11-S499P and rad50-A78T, which we show to specifically affect Tel1 activation without impairing MRX functions in DSB repair. Both Mre11-S499P and Rad50-A78T reduce Tel1–MRX interaction leading to poor Tel1 association at DSBs and consequent loss of Tel1 activation. The Mre11-S499P variant reduces Mre11–Rad50 interaction, suggesting an important role for MR complex formation in Tel1 activation. Molecular dynamics simulations show that the wild type MR subcomplex bound to ATP lingers in a tightly ‘closed’ conformation, while ADP presence leads to the destabilization of Rad50 dimer and of Mre11–Rad50 association, both events being required for MR conformational transition to an open state. By contrast, MRA78T undertakes complex opening even if Rad50 is bound to ATP, indicating that defective Tel1 activation caused by MRA78T results from destabilization of the ATP-bound conformational state." @default.
• W2912175037 created "2019-02-21" @default.
• W2912175037 creator A5004803072 @default.
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• W2912175037 creator A5053058814 @default.
• W2912175037 creator A5057801375 @default.
• W2912175037 creator A5077831719 @default.
• W2912175037 date "2019-01-30" @default.
• W2912175037 modified "2023-10-16" @default.
• W2912175037 title "The ATP-bound conformation of the Mre11–Rad50 complex is essential for Tel1/ATM activation" @default.
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• W2912175037 cites W1973678001 @default.
• W2912175037 cites W1981747417 @default.
• W2912175037 cites W1983218764 @default.
• W2912175037 cites W1992632955 @default.
• W2912175037 cites W1993026474 @default.
• W2912175037 cites W1994954342 @default.
• W2912175037 cites W2002454928 @default.
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• W2912175037 cites W2019016428 @default.
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• W2912175037 cites W2031989940 @default.
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• W2912175037 doi "https://doi.org/10.1093/nar/gkz038" @default.
• W2912175037 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6468247" @default.
• W2912175037 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30698745" @default.
• W2912175037 hasPublicationYear "2019" @default.
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