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• W2912175280 startingPage "277" @default.
• W2912175280 abstract "Aqueous two-phase extraction (ATPE) of R-Phycoerythrin (R-PE), a fluorescent and commercially valuable protein, was carried out in two parallel schemes. In scheme-1, ATPE alone was employed, and in scheme-2, process-integration was attempted, where primary extract was subjected to precipitation before standardization of process parameters of ATPE. Scheme-1 with polyethylene glycol (PEG)-3350/potassium phosphate system has resulted in very low R-PE yield (26% w/w) without much enrichment in purity (0.3). In scheme-2, PEG-1450/potassium phosphate system (pH 6, tie-line length 12.26% and lower volume-ratio) has resulted in higher R-PE purity (0.74) with 72% yield. R-PE containing PEG-rich (top) phase was subjected to ultrafiltration, to remove phase forming components, resulting in further increase in R-PE purity (1.1). Overall enrichment in R-PE purity of 11-fold with 57% (w/w) yield and removal of about 95% of total sugars was achieved in scheme-2. Intactness of R-PE after processing was confirmed by absorbance and emission spectrum analysis." @default.
• W2912175280 created "2019-02-21" @default.
• W2912175280 creator A5046976729 @default.
• W2912175280 creator A5057254732 @default.
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• W2912175280 date "2019-05-01" @default.
• W2912175280 modified "2023-09-29" @default.
• W2912175280 title "Aqueous two-phase extraction of R-Phycoerythrin from marine macro-algae, Gelidium pusillum" @default.
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• W2912175280 doi "https://doi.org/10.1016/j.biortech.2019.02.044" @default.
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