Matches in SemOpenAlex for { <https://semopenalex.org/work/W2912609145> ?p ?o ?g. }
- W2912609145 endingPage "2698" @default.
- W2912609145 startingPage "2681" @default.
- W2912609145 abstract "Most eukaryotic expression systems make use of host-cell nuclear transcriptional and post-transcriptional machineries. Here, we present the first generation of the chimeric cytoplasmic capping-prone phage polymerase (C3P3-G1) expression system developed by biological engineering, which generates capped and polyadenylated transcripts in host-cell cytoplasm by means of two components. First, an artificial single-unit chimeric enzyme made by fusing an mRNA capping enzyme and a DNA-dependent RNA polymerase. Second, specific DNA templates designed to operate with the C3P3-G1 enzyme, which encode for the transcripts and their artificial polyadenylation. This system, which can potentially be adapted to any in cellulo or in vivo eukaryotic expression applications, was optimized for transient expression in mammalian cells. C3P3-G1 shows promising results for protein production in Chinese Hamster Ovary (CHO-K1) cells. This work also provides avenues for enhancing the performances for next generation C3P3 systems." @default.
- W2912609145 created "2019-02-21" @default.
- W2912609145 creator A5009333452 @default.
- W2912609145 creator A5026525163 @default.
- W2912609145 creator A5031298819 @default.
- W2912609145 creator A5031622487 @default.
- W2912609145 creator A5032723831 @default.
- W2912609145 creator A5036587020 @default.
- W2912609145 creator A5039783946 @default.
- W2912609145 creator A5047960752 @default.
- W2912609145 creator A5050373806 @default.
- W2912609145 creator A5054627794 @default.
- W2912609145 creator A5068775377 @default.
- W2912609145 creator A5072378615 @default.
- W2912609145 creator A5073466945 @default.
- W2912609145 creator A5076172595 @default.
- W2912609145 creator A5076330073 @default.
- W2912609145 date "2019-02-06" @default.
- W2912609145 modified "2023-10-15" @default.
- W2912609145 title "C3P3-G1: first generation of a eukaryotic artificial cytoplasmic expression system" @default.
- W2912609145 cites W114406948 @default.
- W2912609145 cites W1531284766 @default.
- W2912609145 cites W1532109734 @default.
- W2912609145 cites W1537763360 @default.
- W2912609145 cites W1538572268 @default.
- W2912609145 cites W1554656840 @default.
- W2912609145 cites W1562437554 @default.
- W2912609145 cites W1573653133 @default.
- W2912609145 cites W1585782442 @default.
- W2912609145 cites W1595801567 @default.
- W2912609145 cites W1596643341 @default.
- W2912609145 cites W1662362821 @default.
- W2912609145 cites W1671005900 @default.
- W2912609145 cites W1849442221 @default.
- W2912609145 cites W1851443011 @default.
- W2912609145 cites W1856422630 @default.
- W2912609145 cites W1964259728 @default.
- W2912609145 cites W1968518211 @default.
- W2912609145 cites W1971902973 @default.
- W2912609145 cites W1973430331 @default.
- W2912609145 cites W1979801688 @default.
- W2912609145 cites W1980892321 @default.
- W2912609145 cites W1981779684 @default.
- W2912609145 cites W1981850714 @default.
- W2912609145 cites W1984998685 @default.
- W2912609145 cites W1989671782 @default.
- W2912609145 cites W1991884238 @default.
- W2912609145 cites W1994542643 @default.
- W2912609145 cites W1996803605 @default.
- W2912609145 cites W2000051603 @default.
- W2912609145 cites W2000193022 @default.
- W2912609145 cites W2000693703 @default.
- W2912609145 cites W2002645517 @default.
- W2912609145 cites W2005411001 @default.
- W2912609145 cites W2008982036 @default.
- W2912609145 cites W2012709143 @default.
- W2912609145 cites W2012958593 @default.
- W2912609145 cites W2013286714 @default.
- W2912609145 cites W2017515700 @default.
- W2912609145 cites W2018364304 @default.
- W2912609145 cites W2021302019 @default.
- W2912609145 cites W2021970306 @default.
- W2912609145 cites W2028322263 @default.
- W2912609145 cites W2038267895 @default.
- W2912609145 cites W2039551908 @default.
- W2912609145 cites W2039863964 @default.
- W2912609145 cites W2050843600 @default.
- W2912609145 cites W2055184396 @default.
- W2912609145 cites W2059973210 @default.
- W2912609145 cites W2060554618 @default.
- W2912609145 cites W2060902122 @default.
- W2912609145 cites W2063924467 @default.
- W2912609145 cites W2076627305 @default.
- W2912609145 cites W2078392705 @default.
- W2912609145 cites W2078964320 @default.
- W2912609145 cites W2082091164 @default.
- W2912609145 cites W2082317854 @default.
- W2912609145 cites W2085223543 @default.
- W2912609145 cites W2086274688 @default.
- W2912609145 cites W2093526175 @default.
- W2912609145 cites W2095950646 @default.
- W2912609145 cites W2096071764 @default.
- W2912609145 cites W2101540294 @default.
- W2912609145 cites W2112531612 @default.
- W2912609145 cites W2115102378 @default.
- W2912609145 cites W2121649903 @default.
- W2912609145 cites W2123990753 @default.
- W2912609145 cites W2128659272 @default.
- W2912609145 cites W2129214225 @default.
- W2912609145 cites W2130685695 @default.
- W2912609145 cites W2136446084 @default.
- W2912609145 cites W2139202053 @default.
- W2912609145 cites W2140893204 @default.
- W2912609145 cites W2141137217 @default.
- W2912609145 cites W2142678478 @default.
- W2912609145 cites W2151978625 @default.
- W2912609145 cites W2159666069 @default.
- W2912609145 cites W2160550953 @default.