FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2945886406> ?p ?o ?g. }

• W2945886406 abstract "Extracellular high mobility group box 1 protein (HMGB1) serves a central role in inflammation as a transporter protein, which binds other immune-activating molecules that are endocytosed via the receptor for advanced glycation end-products (RAGE). These pro-inflammatory complexes are targeted to the endolysosomal compartment, where HMGB1 permeabilizes the lysosomes. This enables HMGB1-partner molecules to avoid degradation, to leak into the cytosol, and to reach cognate immune-activating sensors. Lipopolysaccharide (LPS) requires this pathway to generate pyroptosis by accessing its key cytosolic receptors, murine caspase 11, or the human caspases 4 and 5. This lytic, pro-inflammatory cell death plays a fundamental pathogenic role in gram-negative sepsis. The aim of the study was to identify molecules inhibiting HMGB1 or HMGB1/LPS cellular internalization. Endocytosis was studied in cultured macrophages using Alexa Fluor-labeled HMGB1 or complexes of HMGB1 and Alexa Fluor-labeled LPS in the presence of an anti-HMGB1 monoclonal antibody (mAb), recombinant HMGB1 box A protein, acetylcholine, the nicotinic acetylcholine receptor subtype alpha 7 (α7 nAChR) agonist GTS-21, or a dynamin-specific inhibitor of endocytosis. Images were obtained by fluorescence microscopy and quantified by the ImageJ processing program (NIH). Data were analyzed using student’s t test or one-way ANOVA followed by the least significant difference or Tukey’s tests. Anti-HMGB1 mAb, recombinant HMGB1 antagonist box A protein, acetylcholine, GTS-21, and the dynamin-specific inhibitor of endocytosis inhibited internalization of HMGB1 or HMGB1-LPS complexes in cultured macrophages. These agents prevented macrophage activation in response to HMGB1 and/or HMGB1-LPS complexes. These results demonstrate that therapies based on HMGB1 antagonists and the cholinergic anti-inflammatory pathway share a previously unrecognized molecular mechanism of substantial clinical relevance." @default.
• W2945886406 created "2019-05-29" @default.
• W2945886406 creator A5007010287 @default.
• W2945886406 creator A5008522740 @default.
• W2945886406 creator A5017063310 @default.
• W2945886406 creator A5019646261 @default.
• W2945886406 creator A5044301848 @default.
• W2945886406 creator A5046380471 @default.
• W2945886406 creator A5050770339 @default.
• W2945886406 creator A5052851685 @default.
• W2945886406 creator A5060127395 @default.
• W2945886406 creator A5075492017 @default.
• W2945886406 creator A5077906192 @default.
• W2945886406 creator A5078542779 @default.
• W2945886406 creator A5078561745 @default.
• W2945886406 date "2019-04-11" @default.
• W2945886406 modified "2023-10-17" @default.
• W2945886406 title "Inhibition of HMGB1/RAGE-mediated endocytosis by HMGB1 antagonist box A, anti-HMGB1 antibodies, and cholinergic agonists suppresses inflammation" @default.
• W2945886406 cites W1581387014 @default.
• W2945886406 cites W1598235393 @default.
• W2945886406 cites W1614633287 @default.
• W2945886406 cites W1627944730 @default.
• W2945886406 cites W1672954108 @default.
• W2945886406 cites W1984699561 @default.
• W2945886406 cites W1994190235 @default.
• W2945886406 cites W1996044520 @default.
• W2945886406 cites W2004437604 @default.
• W2945886406 cites W2010008904 @default.
• W2945886406 cites W2021736304 @default.
• W2945886406 cites W2022901113 @default.
• W2945886406 cites W2046775696 @default.
• W2945886406 cites W2064510030 @default.
• W2945886406 cites W2105327109 @default.
• W2945886406 cites W2109334664 @default.
• W2945886406 cites W2112079611 @default.
• W2945886406 cites W2114445825 @default.
• W2945886406 cites W2132959555 @default.
• W2945886406 cites W2134316956 @default.
• W2945886406 cites W2137626178 @default.
• W2945886406 cites W2152022294 @default.
• W2945886406 cites W2154170498 @default.
• W2945886406 cites W2160369507 @default.
• W2945886406 cites W2164714222 @default.
• W2945886406 cites W2280404143 @default.
• W2945886406 cites W2414773926 @default.
• W2945886406 cites W2431987552 @default.
• W2945886406 cites W2478211415 @default.
• W2945886406 cites W2792452770 @default.
• W2945886406 cites W2793077245 @default.
• W2945886406 cites W2802639922 @default.
• W2945886406 cites W2896781864 @default.
• W2945886406 cites W4245060634 @default.
• W2945886406 cites W67488918 @default.
• W2945886406 doi "https://doi.org/10.1186/s10020-019-0081-6" @default.
• W2945886406 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6460792" @default.
• W2945886406 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30975096" @default.
• W2945886406 hasPublicationYear "2019" @default.
• W2945886406 type Work @default.
• W2945886406 sameAs 2945886406 @default.
• W2945886406 citedByCount "68" @default.
• W2945886406 countsByYear W29458864062019 @default.
• W2945886406 countsByYear W29458864062020 @default.
• W2945886406 countsByYear W29458864062021 @default.
• W2945886406 countsByYear W29458864062022 @default.
• W2945886406 countsByYear W29458864062023 @default.
• W2945886406 crossrefType "journal-article" @default.
• W2945886406 hasAuthorship W2945886406A5007010287 @default.
• W2945886406 hasAuthorship W2945886406A5008522740 @default.
• W2945886406 hasAuthorship W2945886406A5017063310 @default.
• W2945886406 hasAuthorship W2945886406A5019646261 @default.
• W2945886406 hasAuthorship W2945886406A5044301848 @default.
• W2945886406 hasAuthorship W2945886406A5046380471 @default.
• W2945886406 hasAuthorship W2945886406A5050770339 @default.
• W2945886406 hasAuthorship W2945886406A5052851685 @default.
• W2945886406 hasAuthorship W2945886406A5060127395 @default.
• W2945886406 hasAuthorship W2945886406A5075492017 @default.
• W2945886406 hasAuthorship W2945886406A5077906192 @default.
• W2945886406 hasAuthorship W2945886406A5078542779 @default.
• W2945886406 hasAuthorship W2945886406A5078561745 @default.
• W2945886406 hasBestOaLocation W29458864061 @default.
• W2945886406 hasConcept C139770010 @default.
• W2945886406 hasConcept C153911025 @default.
• W2945886406 hasConcept C156407911 @default.
• W2945886406 hasConcept C169760540 @default.
• W2945886406 hasConcept C170493617 @default.
• W2945886406 hasConcept C185592680 @default.
• W2945886406 hasConcept C2778857457 @default.
• W2945886406 hasConcept C2780545709 @default.
• W2945886406 hasConcept C28005876 @default.
• W2945886406 hasConcept C55493867 @default.
• W2945886406 hasConcept C86803240 @default.
• W2945886406 hasConcept C95444343 @default.
• W2945886406 hasConceptScore W2945886406C139770010 @default.
• W2945886406 hasConceptScore W2945886406C153911025 @default.
• W2945886406 hasConceptScore W2945886406C156407911 @default.
• W2945886406 hasConceptScore W2945886406C169760540 @default.
• W2945886406 hasConceptScore W2945886406C170493617 @default.
• W2945886406 hasConceptScore W2945886406C185592680 @default.
• W2945886406 hasConceptScore W2945886406C2778857457 @default.
• W2945886406 hasConceptScore W2945886406C2780545709 @default.

• next