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• W2947133669 abstract "ABSTRACT Data independent acquisition (DIA) modes isolate and concurrently fragment populations of different precursors by cycling through segments of a predefined precursor m/z range. Although these selection windows collectively cover the entire m/z range, overall only a few percent of all incoming ions are sampled. Making use of the correlation of molecular weight and ion mobility in a trapped ion mobility device (timsTOF Pro), we here devise a novel scan mode that samples up to 100% of the peptide precursor ion current. We extend an established targeted data extraction workflow by including the ion mobility dimension for both signal extraction and scoring, thereby increasing the specificity for precursor identification. Data acquired from whole proteome digests and mixed organism samples demonstrate deep proteome coverage and a very high degree of reproducibility as well as quantitative accuracy, even from 10 ng sample amounts." @default.
• W2947133669 created "2019-06-07" @default.
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• W2947133669 date "2019-05-31" @default.
• W2947133669 modified "2023-09-30" @default.
• W2947133669 title "Parallel accumulation – serial fragmentation combined with data-independent acquisition (diaPASEF): Bottom-up proteomics with near optimal ion usage" @default.
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• W2947133669 doi "https://doi.org/10.1101/656207" @default.
• W2947133669 hasPublicationYear "2019" @default.
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