FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2950870167> ?p ?o ?g. }

• W2950870167 abstract "Cellulosic biomass has great potential as a renewable biofuel resource. Robust, high-performance enzymes are needed to effectively utilize this valuable resource. In this study, metatranscriptomics was used to explore the carbohydrate-active enzymes (CAZymes), especially glycoside hydrolases (GHs), present in the rumen microbiome of Hu sheep. Select CAZymes were experimentally verified and characterized after cloning and expression in E. coli.The metatranscriptomes of six Hu sheep rumen microbiomes yielded 42.3 Gbp of quality-checked sequence data that represented in total 2,380,783 unigenes after de novo assembling using Trinity and clustered with CD-HIT-EST. Annotation using the CAZy database revealed that 2.65% of the unigenes encoded GHs, which were assigned to 111 different CAZymes families. Firmicutes (18.7%) and Bacteroidetes (13.8%) were the major phyla to which the unigenes were taxonomically assigned. In total, 14,489 unigenes were annotated to 15 cellulase-containing GH families, with GH3, GH5 and GH9 being the predominant. From these putative cellulase-encoding unigenes, 4225 open reading frames (ORFs) were predicted to contain 2151 potential cellulase catalytic modules. Additionally, 147 ORFs were found to encode proteins that contain carbohydrate-binding modules (CBMs). Heterogeneous expression of 30 candidate cDNAs from the GH5 family in E. coli BL21 showed that 17 of the tested proteins had endoglucanase activity, while 7 exhibited exoglucanase activity. Interestingly, two of the GH5 proteins (Cel5A-h28 and Cel5A-h11) showed high specific activity against carboxymethylcellulose (CMC) and p-nitrophenyl-β-d-cellobioside (pNPC) (222.2 and 142.8 U/mg), respectively. The optimal pH value for activity of Cel5A-h11 and Cel5A-h28 was 6.0 for both enzymes, and optimal temperatures were 40 and 50 °C, respectively. Both enzymes retained over 70 and 60%, respectively, of their original activities after incubation at 40 °C for 60 min. However, their activities were rapidly diminished upon exposure to higher temperatures. Cel5A-h11 and Cel5A-h28 retained more than 80 and 60% of their maximal enzymatic activities after incubation for 16 h in buffered solutions in the pH range from 4.0 to 9.0.The metatranscriptomic results revealed that the rumen microbiome of Hu sheep encoded a repertoire of new enzymes capable of cellulose degradation and metatranscriptomics was an effective method to discover novel cellulases for biotechnological applications." @default.
• W2950870167 created "2019-06-27" @default.
• W2950870167 creator A5004396301 @default.
• W2950870167 creator A5038774334 @default.
• W2950870167 creator A5059483665 @default.
• W2950870167 creator A5077925758 @default.
• W2950870167 creator A5078269576 @default.
• W2950870167 date "2019-06-20" @default.
• W2950870167 modified "2023-10-16" @default.
• W2950870167 title "Metatranscriptomics of the Hu sheep rumen microbiome reveals novel cellulases" @default.
• W2950870167 cites W1761770787 @default.
• W2950870167 cites W1943183150 @default.
• W2950870167 cites W1964106002 @default.
• W2950870167 cites W1969346416 @default.
• W2950870167 cites W1971820570 @default.
• W2950870167 cites W1973032291 @default.
• W2950870167 cites W1973561696 @default.
• W2950870167 cites W1989080603 @default.
• W2950870167 cites W1991497480 @default.
• W2950870167 cites W1996053983 @default.
• W2950870167 cites W1996423252 @default.
• W2950870167 cites W1999574084 @default.
• W2950870167 cites W2009674410 @default.
• W2950870167 cites W2011657487 @default.
• W2950870167 cites W2019392185 @default.
• W2950870167 cites W2025983687 @default.
• W2950870167 cites W2072373747 @default.
• W2950870167 cites W2073821309 @default.
• W2950870167 cites W2077883748 @default.
• W2950870167 cites W2084438319 @default.
• W2950870167 cites W2093276678 @default.
• W2950870167 cites W2098839738 @default.
• W2950870167 cites W2106180697 @default.
• W2950870167 cites W2108929776 @default.
• W2950870167 cites W2127319598 @default.
• W2950870167 cites W2128635872 @default.
• W2950870167 cites W2135621733 @default.
• W2950870167 cites W2141458291 @default.
• W2950870167 cites W2143842034 @default.
• W2950870167 cites W2151557512 @default.
• W2950870167 cites W2156074263 @default.
• W2950870167 cites W2162595238 @default.
• W2950870167 cites W2170747616 @default.
• W2950870167 cites W2171533830 @default.
• W2950870167 cites W2280635172 @default.
• W2950870167 cites W2336330109 @default.
• W2950870167 cites W2419364079 @default.
• W2950870167 cites W2556608672 @default.
• W2950870167 cites W2594924779 @default.
• W2950870167 cites W2606024116 @default.
• W2950870167 cites W2607133081 @default.
• W2950870167 cites W2610003283 @default.
• W2950870167 cites W2715054119 @default.
• W2950870167 cites W2765229221 @default.
• W2950870167 cites W2786358949 @default.
• W2950870167 cites W2809402915 @default.
• W2950870167 cites W2904299670 @default.
• W2950870167 cites W4238673257 @default.
• W2950870167 cites W4293247451 @default.
• W2950870167 doi "https://doi.org/10.1186/s13068-019-1498-4" @default.
• W2950870167 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6587244" @default.
• W2950870167 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31249617" @default.
• W2950870167 hasPublicationYear "2019" @default.
• W2950870167 type Work @default.
• W2950870167 sameAs 2950870167 @default.
• W2950870167 citedByCount "38" @default.
• W2950870167 countsByYear W29508701672020 @default.
• W2950870167 countsByYear W29508701672021 @default.
• W2950870167 countsByYear W29508701672022 @default.
• W2950870167 countsByYear W29508701672023 @default.
• W2950870167 crossrefType "journal-article" @default.
• W2950870167 hasAuthorship W2950870167A5004396301 @default.
• W2950870167 hasAuthorship W2950870167A5038774334 @default.
• W2950870167 hasAuthorship W2950870167A5059483665 @default.
• W2950870167 hasAuthorship W2950870167A5077925758 @default.
• W2950870167 hasAuthorship W2950870167A5078269576 @default.
• W2950870167 hasBestOaLocation W29508701671 @default.
• W2950870167 hasConcept C100544194 @default.
• W2950870167 hasConcept C104317684 @default.
• W2950870167 hasConcept C141603559 @default.
• W2950870167 hasConcept C143121216 @default.
• W2950870167 hasConcept C15151743 @default.
• W2950870167 hasConcept C167625842 @default.
• W2950870167 hasConcept C196032511 @default.
• W2950870167 hasConcept C2777385719 @default.
• W2950870167 hasConcept C2777695942 @default.
• W2950870167 hasConcept C2779251873 @default.
• W2950870167 hasConcept C2779676784 @default.
• W2950870167 hasConcept C2780027724 @default.
• W2950870167 hasConcept C2780412358 @default.
• W2950870167 hasConcept C2780530800 @default.
• W2950870167 hasConcept C31903555 @default.
• W2950870167 hasConcept C42062724 @default.
• W2950870167 hasConcept C47289529 @default.
• W2950870167 hasConcept C54355233 @default.
• W2950870167 hasConcept C55493867 @default.
• W2950870167 hasConcept C59822182 @default.
• W2950870167 hasConcept C70262065 @default.
• W2950870167 hasConcept C86803240 @default.
• W2950870167 hasConcept C89423630 @default.

• next