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• W2958743669 abstract "Abstract In the last decade, a technological shift in the bioinformatics field has occurred: larger genomes can now be sequenced quickly and cost effectively, resulting in the computational need to efficiently compare large and abundant sequences. Furthermore, detecting conserved similarities across large collections of genomes remains a problem. The size of chromosomes, along with the substantial amount of noise and number of repeats found in DNA sequences (particularly in mammals and plants), leads to a scenario where executing and waiting for complete outputs is both time and resource consuming. Filtering steps, manual examination and annotation, very long execution times and a high demand for computational resources represent a few of the many difficulties faced in large genome comparisons. In this work, we provide a method designed for comparisons of considerable amounts of very long sequences that employs a heuristic algorithm capable of separating noise and repeats from conserved fragments in pairwise genomic comparisons. We provide software implementation that computes in linear time using one core as a minimum and a small, constant memory footprint. The method produces both a previsualization of the comparison and a collection of indices to drastically reduce computational complexity when performing exhaustive comparisons. Last, the method scores the comparison to automate classification of sequences and produces a list of detected synteny blocks to enable new evolutionary studies." @default.
• W2958743669 created "2019-07-23" @default.
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• W2958743669 date "2019-07-16" @default.
• W2958743669 modified "2023-10-14" @default.
• W2958743669 title "Ultra-fast genome comparison for large-scale genomic experiments" @default.
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• W2958743669 doi "https://doi.org/10.1038/s41598-019-46773-w" @default.
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