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- W3049367025 abstract "Abstract Background Although cord blood (CB) offers promise for treatment of patients with high-risk hematological malignancies and immune disorders, the limited numbers of hematopoietic stem cell (HSC)/progenitor cell in a CB unit and straitened circumstances in expanding ex vivo make it quite challenging to develop the successful cell therapies. Methods In this study, a novel strategy has been developed to support ex vivo expansion of hematopoietic stem and progenitor cells (HSPCs) by coculture with engineered human umbilical arterial endothelial cells (HuAECs-E4orf1-GFP), which expresses E4ORF1 stably by using a retroviral system. Results Coculture of CD34 + hCB cells with HuAECs-E4orf1-GFP resulted in generation of considerably more total nucleated cells, CD34 + CD38 − , and CD34 + CD38 − CD90 + HSPCs in comparison with that of cytokines alone or that of coculture with human umbilical vein endothelial cells (HuVECs) after 14-day amplification. The in vitro multilineage differentiation potential and in vivo repopulating capacity of the expanded hematopoietic cells cocultured with HuAECs-E4orf1-GFP were also markedly enhanced compared with the other two control groups. DLL4, a major determinant of arterial endothelial cell (EC) identity, was associated with CD34 + hCB cells amplified on HuAECs-E4orf1-GFP. Conclusions Collectively, we demonstrated that HuAECs acted as a permissive niche in facilitating expansion of HSPCs. Our study further implicated that the crucial factors and related pathways presented in HuAECs may give a hint to maintain self-renewal of bona fide HSCs." @default.
- W3049367025 created "2020-08-21" @default.
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- W3049367025 date "2020-08-14" @default.
- W3049367025 modified "2023-10-11" @default.
- W3049367025 title "Arterial endothelium creates a permissive niche for expansion of human cord blood hematopoietic stem and progenitor cells" @default.
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- W3049367025 doi "https://doi.org/10.1186/s13287-020-01880-8" @default.
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