Matches in SemOpenAlex for { <https://semopenalex.org/work/W3087132608> ?p ?o ?g. }
- W3087132608 abstract "Abstract O -Acetylation of the capsular polysaccharide (CPS) of Neisseria meningitidis serogroup A (NmA) is critical for the induction of functional immune responses, making this modification mandatory for CPS-based anti-NmA vaccines. Using comprehensive NMR studies, we demonstrate that O -acetylation stabilizes the labile anomeric phosphodiester-linkages of the NmA-CPS and occurs in position C3 and C4 of the N -acetylmannosamine units due to enzymatic transfer and non-enzymatic ester migration, respectively. To shed light on the enzymatic transfer mechanism, we solved the crystal structure of the capsule O -acetyltransferase CsaC in its apo and acceptor-bound form and of the CsaC-H228A mutant as trapped acetyl-enzyme adduct in complex with CoA. Together with the results of a comprehensive mutagenesis study, the reported structures explain the strict regioselectivity of CsaC and provide insight into the catalytic mechanism, which relies on an unexpected Gln-extension of a classical Ser-His-Asp triad, embedded in an α/β-hydrolase fold." @default.
- W3087132608 created "2020-09-25" @default.
- W3087132608 creator A5015595095 @default.
- W3087132608 creator A5018164726 @default.
- W3087132608 creator A5031654186 @default.
- W3087132608 creator A5034207673 @default.
- W3087132608 creator A5041772630 @default.
- W3087132608 creator A5053231718 @default.
- W3087132608 creator A5060218108 @default.
- W3087132608 creator A5064722288 @default.
- W3087132608 creator A5069761011 @default.
- W3087132608 creator A5071184533 @default.
- W3087132608 creator A5079172960 @default.
- W3087132608 date "2020-09-18" @default.
- W3087132608 modified "2023-10-18" @default.
- W3087132608 title "Structural and mechanistic basis of capsule O-acetylation in Neisseria meningitidis serogroup A" @default.
- W3087132608 cites W1591660320 @default.
- W3087132608 cites W1808949860 @default.
- W3087132608 cites W1968507919 @default.
- W3087132608 cites W1972885220 @default.
- W3087132608 cites W1975317507 @default.
- W3087132608 cites W1979640591 @default.
- W3087132608 cites W1981494855 @default.
- W3087132608 cites W1981894568 @default.
- W3087132608 cites W1989923722 @default.
- W3087132608 cites W1994893698 @default.
- W3087132608 cites W1995278899 @default.
- W3087132608 cites W2003358759 @default.
- W3087132608 cites W2003601780 @default.
- W3087132608 cites W2005977813 @default.
- W3087132608 cites W2007028946 @default.
- W3087132608 cites W2007855505 @default.
- W3087132608 cites W2014078475 @default.
- W3087132608 cites W2019425225 @default.
- W3087132608 cites W2021251655 @default.
- W3087132608 cites W2029370732 @default.
- W3087132608 cites W2030517878 @default.
- W3087132608 cites W2041969493 @default.
- W3087132608 cites W2055264790 @default.
- W3087132608 cites W2067964678 @default.
- W3087132608 cites W2071866439 @default.
- W3087132608 cites W2082309459 @default.
- W3087132608 cites W2097815621 @default.
- W3087132608 cites W2108923073 @default.
- W3087132608 cites W2111290761 @default.
- W3087132608 cites W2113220501 @default.
- W3087132608 cites W2120772351 @default.
- W3087132608 cites W2127322768 @default.
- W3087132608 cites W2127821227 @default.
- W3087132608 cites W2132760372 @default.
- W3087132608 cites W2140630847 @default.
- W3087132608 cites W2141413828 @default.
- W3087132608 cites W2142819552 @default.
- W3087132608 cites W2163341755 @default.
- W3087132608 cites W2169859589 @default.
- W3087132608 cites W2169947235 @default.
- W3087132608 cites W2263196200 @default.
- W3087132608 cites W2266791999 @default.
- W3087132608 cites W2323883449 @default.
- W3087132608 cites W2414307244 @default.
- W3087132608 cites W2471981404 @default.
- W3087132608 cites W2518529034 @default.
- W3087132608 cites W2521039464 @default.
- W3087132608 cites W2522253280 @default.
- W3087132608 cites W2605797771 @default.
- W3087132608 cites W2610479233 @default.
- W3087132608 cites W2765799080 @default.
- W3087132608 cites W2766116057 @default.
- W3087132608 cites W2783201393 @default.
- W3087132608 cites W2795776656 @default.
- W3087132608 cites W2799524357 @default.
- W3087132608 cites W2805253599 @default.
- W3087132608 cites W2807081433 @default.
- W3087132608 cites W2809276770 @default.
- W3087132608 cites W2853967274 @default.
- W3087132608 cites W2905904940 @default.
- W3087132608 cites W2909049629 @default.
- W3087132608 cites W2980918893 @default.
- W3087132608 cites W2980984934 @default.
- W3087132608 cites W3009831659 @default.
- W3087132608 cites W3023578215 @default.
- W3087132608 cites W4239814272 @default.
- W3087132608 cites W4248872320 @default.
- W3087132608 doi "https://doi.org/10.1038/s41467-020-18464-y" @default.
- W3087132608 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7501274" @default.
- W3087132608 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32948778" @default.
- W3087132608 hasPublicationYear "2020" @default.
- W3087132608 type Work @default.
- W3087132608 sameAs 3087132608 @default.
- W3087132608 citedByCount "10" @default.
- W3087132608 countsByYear W30871326082021 @default.
- W3087132608 countsByYear W30871326082022 @default.
- W3087132608 countsByYear W30871326082023 @default.
- W3087132608 crossrefType "journal-article" @default.
- W3087132608 hasAuthorship W3087132608A5015595095 @default.
- W3087132608 hasAuthorship W3087132608A5018164726 @default.
- W3087132608 hasAuthorship W3087132608A5031654186 @default.
- W3087132608 hasAuthorship W3087132608A5034207673 @default.
- W3087132608 hasAuthorship W3087132608A5041772630 @default.
- W3087132608 hasAuthorship W3087132608A5053231718 @default.