FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3100404999> ?p ?o ?g. }

• W3100404999 abstract "ABSTRACT The ATP binding cassette (ABC) family of transporters move diverse small molecules across membranes in nearly all organisms. Transport activity requires conformational switching between inward-facing and outward-facing states driven by ATP-dependent dimerization of two nucleotide binding domains (NBDs). The allosteric mechanism that connects ATP binding and hydrolysis in the NBDs to conformational changes in a substrate binding site in the transmembrane domains (TMDs) presents an unresolved question. Here we use sequence coevolution analyses together with biochemical characterization to investigate the role of a highly conserved motif called the peptide sensor in coordinating domain rearrangements in the heterodimeric peptide exporter from Thermus thermophilus , TmrAB. Mutations in the peptide sensor motif alter ATP hydrolysis rates as well as substrate release. Disulfide crosslinking, evolutionary trace, and evolutionary coupling analysis reveal that these effects likely destabilize a network between the peptide sensor motif and the Q-loop and X-loop, two known allosteric elements in the NBDs. We further find that disruption of this network in TmrA versus TmrB has different functional consequences, hinting at an intrinsic asymmetry in heterodimeric ABC transporters extending beyond that of the NBDs. These results support a mechanism in which the peptide sensor motifs help coordinate the transition of TmrAB to an outward open conformation, and each half of the transporter likely plays a different role in the conformational cycle of TmrAB." @default.
• W3100404999 created "2020-11-23" @default.
• W3100404999 creator A5001653650 @default.
• W3100404999 creator A5029389368 @default.
• W3100404999 creator A5030273216 @default.
• W3100404999 creator A5036859117 @default.
• W3100404999 creator A5037170067 @default.
• W3100404999 date "2020-01-14" @default.
• W3100404999 modified "2023-10-18" @default.
• W3100404999 title "The peptide sensor motif stabilizes the outward-facing conformation of TmrAB" @default.
• W3100404999 cites W1504187995 @default.
• W3100404999 cites W1981109070 @default.
• W3100404999 cites W1982924924 @default.
• W3100404999 cites W1983518163 @default.
• W3100404999 cites W1986639571 @default.
• W3100404999 cites W1989912462 @default.
• W3100404999 cites W1991208799 @default.
• W3100404999 cites W1994265289 @default.
• W3100404999 cites W1994348363 @default.
• W3100404999 cites W1998093640 @default.
• W3100404999 cites W2001216837 @default.
• W3100404999 cites W2004877959 @default.
• W3100404999 cites W2026984793 @default.
• W3100404999 cites W2030632688 @default.
• W3100404999 cites W2042256502 @default.
• W3100404999 cites W2050912869 @default.
• W3100404999 cites W2051545676 @default.
• W3100404999 cites W2083816365 @default.
• W3100404999 cites W2087944803 @default.
• W3100404999 cites W2093612804 @default.
• W3100404999 cites W2098142549 @default.
• W3100404999 cites W2102762507 @default.
• W3100404999 cites W2104622728 @default.
• W3100404999 cites W2111064199 @default.
• W3100404999 cites W2111935912 @default.
• W3100404999 cites W2114850508 @default.
• W3100404999 cites W2127774996 @default.
• W3100404999 cites W2131299583 @default.
• W3100404999 cites W2135228046 @default.
• W3100404999 cites W2137991504 @default.
• W3100404999 cites W2138122982 @default.
• W3100404999 cites W2140744545 @default.
• W3100404999 cites W2141052558 @default.
• W3100404999 cites W2143325677 @default.
• W3100404999 cites W2151209811 @default.
• W3100404999 cites W2156125289 @default.
• W3100404999 cites W2158266834 @default.
• W3100404999 cites W2160378127 @default.
• W3100404999 cites W2160903751 @default.
• W3100404999 cites W2161983628 @default.
• W3100404999 cites W2162574056 @default.
• W3100404999 cites W2168974130 @default.
• W3100404999 cites W2170734806 @default.
• W3100404999 cites W2177099235 @default.
• W3100404999 cites W2570779740 @default.
• W3100404999 cites W2589923912 @default.
• W3100404999 cites W2597231659 @default.
• W3100404999 cites W2763876266 @default.
• W3100404999 cites W2766922062 @default.
• W3100404999 cites W2777934057 @default.
• W3100404999 cites W2783571553 @default.
• W3100404999 cites W2885134480 @default.
• W3100404999 cites W2887263514 @default.
• W3100404999 cites W2944923223 @default.
• W3100404999 cites W2957093526 @default.
• W3100404999 cites W2969458429 @default.
• W3100404999 cites W2969896272 @default.
• W3100404999 cites W2972411752 @default.
• W3100404999 cites W3016474366 @default.
• W3100404999 cites W3028911473 @default.
• W3100404999 cites W4252314477 @default.
• W3100404999 doi "https://doi.org/10.1101/2020.01.12.903617" @default.
• W3100404999 hasPublicationYear "2020" @default.
• W3100404999 type Work @default.
• W3100404999 sameAs 3100404999 @default.
• W3100404999 citedByCount "0" @default.
• W3100404999 crossrefType "posted-content" @default.
• W3100404999 hasAuthorship W3100404999A5001653650 @default.
• W3100404999 hasAuthorship W3100404999A5029389368 @default.
• W3100404999 hasAuthorship W3100404999A5030273216 @default.
• W3100404999 hasAuthorship W3100404999A5036859117 @default.
• W3100404999 hasAuthorship W3100404999A5037170067 @default.
• W3100404999 hasBestOaLocation W31004049991 @default.
• W3100404999 hasConcept C104317684 @default.
• W3100404999 hasConcept C113027372 @default.
• W3100404999 hasConcept C117745874 @default.
• W3100404999 hasConcept C118892022 @default.
• W3100404999 hasConcept C12554922 @default.
• W3100404999 hasConcept C139425391 @default.
• W3100404999 hasConcept C141315368 @default.
• W3100404999 hasConcept C149011108 @default.
• W3100404999 hasConcept C166342909 @default.
• W3100404999 hasConcept C181199279 @default.
• W3100404999 hasConcept C185592680 @default.
• W3100404999 hasConcept C23265538 @default.
• W3100404999 hasConcept C2777339483 @default.
• W3100404999 hasConcept C2779281246 @default.
• W3100404999 hasConcept C2780883830 @default.
• W3100404999 hasConcept C41625074 @default.
• W3100404999 hasConcept C44312359 @default.

• next