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• W311928546 abstract "Objective: To determine the effect of leukemia inhibitory factor (LIF) on the quality of in vitro produced mouse blastocyst and the efficiency of embryonic stem (ES) cell derivation. Design: Experimental study Setting: Reproductive Medicine Unit, Department of Obstetrics and Gynecology, Faculty of Medicine, Chulalongkorn University Material and Method: In vivo fertilized zygotes were collected and subjected to in vitro culture in potassium simplex optimized medium (KSOM) containing 1,000 unit/ml LIF. The developmental ability of the zygote to blastocyst-stage and the cell numbers in blastocysts were evaluated. Expanded blastocysts developed in different culture media were subsequently subjected to ES cell derivation. Main Outcome Measure (s): The influence of LIF on the quality of and the total cell numbers of blastocyst developed in vitro. Results: Supplementation of LIF in KSOM increased the rate of hatching blastocysts (63.8% vs. 53.7%; p 0.05). Established ES cell lines showed typical colony and characteristics of pluripotent murine ES cells. Conclusion: LIF improved the quality of in vitro produced blastocysts but not enhanced ES cell derivation. Keywords: LIF, in vitro, Blastocyst, Embryonic stem cells" @default.
• W311928546 created "2016-06-24" @default.
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• W311928546 date "2008-05-01" @default.
• W311928546 modified "2023-09-24" @default.
• W311928546 title "Effect of leukemia inhibitory factor (LIF) on the quality of in vitro produced mouse blastocysts and subsequent derivation of embryonic stem (ES) cells." @default.
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