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- W3148776095 abstract "Virus infection in host cells is a complex process comprising a large number of dynamic molecular events. Single-virus tracking is a versatile technique to study these events. To perform this technique, viruses must be fluorescently labeled to be visible to fluorescence microscopes. The quantum dot is a kind of fluorescent tag that has many unique optical properties. It has been widely used to label proteins in single-molecule-tracking studies but rarely used to study virus infection, mainly due to the lack of an accepted labeling method. In this study, we developed a lipid-specific method to readily, mildly, specifically, and efficiently label enveloped viruses with quantum dots by recognizing viral envelope lipids with lipid-biotin conjugates and recognizing these lipid-biotin conjugates with streptavidin-quantum dot conjugates. It is not only applicable to normal viruses, but also competent to label the key protein-mutated viruses and the inactivated highly virulent viruses, providing a powerful tool for single-virus tracking." @default.
- W3148776095 created "2021-04-13" @default.
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- W3148776095 date "2020-06-30" @default.
- W3148776095 modified "2023-09-23" @default.
- W3148776095 title "Lipid-Specific Labeling of Enveloped Viruses with Quantum Dots for Single-Virus Tracking" @default.
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- W3148776095 doi "https://doi.org/10.1128/mbio.00135-20" @default.
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