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• W3211855872 abstract "8-oxoguanine DNA glycosylase (OGG), which plays a crucial role in base excision repair (BER), is an important biomarker. The existing highly sensitive fluorescent methods always need complicated amplification design. The method with high sensitivity and simple design at the same time is urgently needed. Here, we developed a highly sensitive detection method for OGG detection with lambda exonuclease and the background signal suppression probe. Through probe structure design, the steric hindrance and competitive binding effects successfully suppressed the background signal. We achieved sensitive detection of OGG with a simple design, and the limit of detection was 5.0 × 10 −4 U mL −1 . Moreover, the method was highly selective and successfully applied to OGG detection in biological samples, which shows the potential clinical application value. • A background suppression strategy was designed for DNA glycosylase (OGG) detection. • The steric hindrance and competitive binding were used in the DNA structure design. • The strategy enabled OGG detection with high sensitivity using a simple design. • The method was applied to OGG detection in complex biological samples." @default.
• W3211855872 created "2021-11-22" @default.
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• W3211855872 date "2021-08-01" @default.
• W3211855872 modified "2023-09-24" @default.
• W3211855872 title "Development of a background signal suppression probe for 8-oxoguanine DNA glycosylase detection" @default.
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• W3211855872 doi "https://doi.org/10.1016/j.aca.2021.338741" @default.
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