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• W4210593651 abstract "The molecular machinery of life is founded on chiral building blocks, but no experimental technique is currently available to distinguish or monitor chiral systems in live cell bio-imaging studies. Luminescent chiral molecules encode a unique optical fingerprint within emitted circularly polarized light (CPL) carrying information about the molecular environment, conformation, and binding state. Here, we present a CPL Laser Scanning Confocal Microscope (CPL-LSCM) capable of simultaneous chiroptical contrast based live-cell imaging of endogenous and engineered CPL-active cellular probes. Further, we demonstrate that CPL-active probes can be activated using two-photon excitation, with complete CPL spectrum recovery. The combination of these two milestone results empowers the multidisciplinary imaging community, allowing the study of chiral interactions on a sub-cellular level in a new (chiral) light." @default.
• W4210593651 created "2022-02-08" @default.
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• W4210593651 date "2022-01-27" @default.
• W4210593651 modified "2023-10-06" @default.
• W4210593651 title "Circularly polarised luminescence laser scanning confocal microscopy to study live cell chiral molecular interactions" @default.
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• W4210593651 doi "https://doi.org/10.1038/s41467-022-28220-z" @default.
• W4210593651 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35087047" @default.
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