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- W4220698695 abstract "Metalloproteins are involved in key cell processes such as photosynthesis, respiration, and oxygen transport. However, the presence of transition metals (notably iron as a component of [Fe-S] clusters) often makes these proteins sensitive to oxygen-induced degradation. Consequently, their study usually requires strict anaerobic conditions. Although X-ray crystallography has been the method of choice for solving macromolecular structures for many years, recently electron microscopy has also become an increasingly powerful structure-solving technique. We have used our previous experience with cryo-crystallography to develop a method to prepare cryo-EM grids in an anaerobic chamber and have applied it to solve the structures of apoferritin and the 3 [Fe4S4]-containing pyruvate ferredoxin oxidoreductase (PFOR) at 2.40 Å and 2.90 Å resolution, respectively. The maps are of similar quality to the ones obtained under air, thereby validating our method as an improvement in the structural investigation of oxygen-sensitive metalloproteins by cryo-EM." @default.
- W4220698695 created "2022-04-03" @default.
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- W4220698695 date "2022-03-12" @default.
- W4220698695 modified "2023-10-14" @default.
- W4220698695 title "Oxygen-Sensitive Metalloprotein Structure Determination by Cryo-Electron Microscopy" @default.
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- W4220698695 doi "https://doi.org/10.3390/biom12030441" @default.
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