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• W4223933071 abstract "In the present work, we developed and evaluated the performance of a new flow cytometry-based single platform, referred to as FC-Duplex IgG1 (HTLV-1/2), for universal and differential serodiagnosis of HTLV-1/2 infection. The proposed technology employs a system for detection of IgG1 antibodies in a single competitive immunofluorescence platform by flow cytometry using fluorescently labeled MT-2/MoT cell line mix coupled to a highly sensitive development system (Biotin/Streptavidin/Phycoerythrin). The stability of fluorescent labeling and the antigenicity of MT-2 and MoT cell lines were confirmed upon storage at -20°C for 2, 6, and 12 months. The anti-HTLV-1/2 IgG1 reactivity, expressed as percentage of positive fluorescent cells (PPFC), was evaluated for each target antigen along the titration curve of test serum samples (1:32 to 1:4,096). Upon selection of target cell line and serum dilutions with higher segregation score between groups, the performance of FIX and FIX & PERM protocols was evaluated. The FIX protocol presented excellent performance indices (Se = 92%/Sp = 94%/AUC = 0.96; Se = 96%/Sp = 100%/AUC = 0.99) for the universal (HTLV-1/2 vs. NI) and differential (HTLV-1 vs. HTLV-2) diagnosis of HTLV-1 infection, respectively. Optimization of the FIX protocol using the principle of synchronous and asynchronous pairwise analysis further improved the performance of FC-Duplex IgG1 (HTLV-1/2), using the FIX protocol for differential diagnosis of HTLV-1 and HTLV-2 infections (Se = 100%/Sp = 100%/AUC = 1.00). In conclusion, the FC-Duplex IgG1 (HTLV-1/2) method represents an innovation in the biotechnology segment with the potential to compose a serological kit for differential diagnosis of HTLV-1/2 infection for reference laboratories and blood centers." @default.
• W4223933071 created "2022-04-19" @default.
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• W4223933071 date "2022-04-14" @default.
• W4223933071 modified "2023-10-16" @default.
• W4223933071 title "A New Flow Cytometry-Based Single Platform for Universal and Differential Serodiagnosis of HTLV-1/2 Infection" @default.
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• W4223933071 doi "https://doi.org/10.3389/fimmu.2022.795815" @default.
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