Matches in SemOpenAlex for { <https://semopenalex.org/work/W4289783418> ?p ?o ?g. }
- W4289783418 endingPage "1124" @default.
- W4289783418 startingPage "1115" @default.
- W4289783418 abstract "Cell-to-cell signaling, or quorum sensing (QS), in many Gram-negative bacteria is governed by small molecule signals (N-acyl-l-homoserine lactones, AHLs) and their cognate receptors (LuxR-type proteins). The mechanistic underpinnings of QS in these bacteria are severely limited due to the challenges of isolating and manipulating most LuxR-type proteins. Reports of quantitative direct-binding experiments on LuxR-type proteins are scarce, and robust and generalizable methods that provide such data are largely nonexistent. We report herein a Förster resonance energy transfer (FRET) assay that leverages (1) conserved tryptophans located in the LuxR-type protein ligand-binding site and synthetic fluorophore–AHL conjugates, and (2) isolation of the proteins bound to weak agonists. The FRET assay permits straightforward measurement of ligand-binding affinities with receptor—either in vitro or in cells—and was shown to be compatible with six LuxR-type proteins. These methods will advance fundamental investigations of LuxR-type protein mechanism and the development of small molecule QS modulators. A FRET-based assay using the conserved tryptophans of bacterial quorum-sensing LuxR-type proteins and synthetic fluorophore-acyl-homoserine lactone conjugates enables measurement of ligand-binding affinities either in vitro or in cells." @default.
- W4289783418 created "2022-08-04" @default.
- W4289783418 creator A5016222491 @default.
- W4289783418 creator A5051106587 @default.
- W4289783418 creator A5068984602 @default.
- W4289783418 creator A5070464655 @default.
- W4289783418 creator A5071821542 @default.
- W4289783418 creator A5072500902 @default.
- W4289783418 creator A5088189732 @default.
- W4289783418 date "2022-08-04" @default.
- W4289783418 modified "2023-10-15" @default.
- W4289783418 title "Autoinducer-fluorophore conjugates enable FRET in LuxR proteins in vitro and in cells" @default.
- W4289783418 cites W1502461125 @default.
- W4289783418 cites W1585864117 @default.
- W4289783418 cites W1964466122 @default.
- W4289783418 cites W1968549820 @default.
- W4289783418 cites W1974441697 @default.
- W4289783418 cites W1982281924 @default.
- W4289783418 cites W1983200428 @default.
- W4289783418 cites W1987516720 @default.
- W4289783418 cites W1988153827 @default.
- W4289783418 cites W1991001144 @default.
- W4289783418 cites W1993810129 @default.
- W4289783418 cites W1997128173 @default.
- W4289783418 cites W2000106654 @default.
- W4289783418 cites W2000591221 @default.
- W4289783418 cites W2008818963 @default.
- W4289783418 cites W2010959934 @default.
- W4289783418 cites W2016995075 @default.
- W4289783418 cites W2020361547 @default.
- W4289783418 cites W2021002410 @default.
- W4289783418 cites W2031700270 @default.
- W4289783418 cites W2032166531 @default.
- W4289783418 cites W2048843475 @default.
- W4289783418 cites W2061433174 @default.
- W4289783418 cites W2061520106 @default.
- W4289783418 cites W2067566372 @default.
- W4289783418 cites W2081148004 @default.
- W4289783418 cites W2089932939 @default.
- W4289783418 cites W2113773263 @default.
- W4289783418 cites W2126922170 @default.
- W4289783418 cites W2127128579 @default.
- W4289783418 cites W2133580858 @default.
- W4289783418 cites W2144150209 @default.
- W4289783418 cites W2154484559 @default.
- W4289783418 cites W2162160645 @default.
- W4289783418 cites W2194852128 @default.
- W4289783418 cites W2415502859 @default.
- W4289783418 cites W2423271305 @default.
- W4289783418 cites W2565819643 @default.
- W4289783418 cites W2770252239 @default.
- W4289783418 cites W2790465394 @default.
- W4289783418 cites W2803604812 @default.
- W4289783418 cites W2884031102 @default.
- W4289783418 cites W2885903823 @default.
- W4289783418 cites W2896857795 @default.
- W4289783418 cites W2914916885 @default.
- W4289783418 cites W2921774375 @default.
- W4289783418 cites W2974013560 @default.
- W4289783418 cites W3005053389 @default.
- W4289783418 cites W3010893115 @default.
- W4289783418 cites W3097510384 @default.
- W4289783418 cites W4247205340 @default.
- W4289783418 doi "https://doi.org/10.1038/s41589-022-01089-1" @default.
- W4289783418 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35927585" @default.
- W4289783418 hasPublicationYear "2022" @default.
- W4289783418 type Work @default.
- W4289783418 citedByCount "0" @default.
- W4289783418 crossrefType "journal-article" @default.
- W4289783418 hasAuthorship W4289783418A5016222491 @default.
- W4289783418 hasAuthorship W4289783418A5051106587 @default.
- W4289783418 hasAuthorship W4289783418A5068984602 @default.
- W4289783418 hasAuthorship W4289783418A5070464655 @default.
- W4289783418 hasAuthorship W4289783418A5071821542 @default.
- W4289783418 hasAuthorship W4289783418A5072500902 @default.
- W4289783418 hasAuthorship W4289783418A5088189732 @default.
- W4289783418 hasBestOaLocation W42897834182 @default.
- W4289783418 hasConcept C104317684 @default.
- W4289783418 hasConcept C118687296 @default.
- W4289783418 hasConcept C121332964 @default.
- W4289783418 hasConcept C170493617 @default.
- W4289783418 hasConcept C185592680 @default.
- W4289783418 hasConcept C2778617687 @default.
- W4289783418 hasConcept C51639874 @default.
- W4289783418 hasConcept C55493867 @default.
- W4289783418 hasConcept C60987743 @default.
- W4289783418 hasConcept C62520636 @default.
- W4289783418 hasConcept C85196483 @default.
- W4289783418 hasConcept C86803240 @default.
- W4289783418 hasConcept C91881484 @default.
- W4289783418 hasConcept C95282255 @default.
- W4289783418 hasConcept C95444343 @default.
- W4289783418 hasConcept C96305047 @default.
- W4289783418 hasConceptScore W4289783418C104317684 @default.
- W4289783418 hasConceptScore W4289783418C118687296 @default.
- W4289783418 hasConceptScore W4289783418C121332964 @default.
- W4289783418 hasConceptScore W4289783418C170493617 @default.
- W4289783418 hasConceptScore W4289783418C185592680 @default.