FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4307309199> ?p ?o ?g. }

• W4307309199 endingPage "e0276285" @default.
• W4307309199 startingPage "e0276285" @default.
• W4307309199 abstract "Protein posttranslational modifications (PTMs) by O-GlcNAc globally rise during pressure-overload hypertrophy (POH). However, a major knowledge gap exists on the specific proteins undergoing changes in O-GlcNAc levels during POH primarily because this PTM is low abundance and easily lost during standard mass spectrometry (MS) conditions used for protein identification. Methodologies have emerged to enrich samples for O-GlcNAcylated proteins prior to MS analysis. Accordingly, our goal was to identify the specific proteins undergoing changes in O-GlcNAc levels during POH. We used C57/Bl6 mice subjected to Sham or transverse aortic constriction (TAC) to create POH. From the hearts, we labelled the O-GlcNAc moiety with tetramethylrhodamine azide (TAMRA) before sample enrichment by TAMRA immunoprecipitation (IP). We used LC-MS/MS to identify and quantify the captured putative O-GlcNAcylated proteins. We identified a total of 700 putative O-GlcNAcylated proteins in Sham and POH. Two hundred thirty-three of these proteins had significantly increased enrichment in POH over Sham suggesting higher O-GlcNAc levels whereas no proteins were significantly decreased by POH. We examined two MS identified metabolic enzymes, CPT1B and the PDH complex, to validate by immunoprecipitation. We corroborated increased O-GlcNAc levels during POH for CPT1B and the PDH complex. Enzyme activity assays suggests higher O-GlcNAcylation increases CPT1 activity and decreases PDH activity during POH. In summary, we generated the first comprehensive list of proteins with putative changes in O-GlcNAc levels during POH. Our results demonstrate the large number of potential proteins and cellular processes affected by O-GlcNAc and serve as a guide for testing specific O-GlcNAc-regulated mechanisms during POH." @default.
• W4307309199 created "2022-10-31" @default.
• W4307309199 creator A5002707544 @default.
• W4307309199 creator A5029387280 @default.
• W4307309199 creator A5034256236 @default.
• W4307309199 creator A5050697789 @default.
• W4307309199 creator A5055812269 @default.
• W4307309199 creator A5071892540 @default.
• W4307309199 creator A5073352935 @default.
• W4307309199 date "2022-10-26" @default.
• W4307309199 modified "2023-09-25" @default.
• W4307309199 title "First comprehensive identification of cardiac proteins with putative increased O-GlcNAc levels during pressure overload hypertrophy" @default.
• W4307309199 cites W1652316604 @default.
• W4307309199 cites W1981809260 @default.
• W4307309199 cites W2016254275 @default.
• W4307309199 cites W2031957765 @default.
• W4307309199 cites W2044496493 @default.
• W4307309199 cites W2055477527 @default.
• W4307309199 cites W2057474602 @default.
• W4307309199 cites W2061011762 @default.
• W4307309199 cites W2062383366 @default.
• W4307309199 cites W2107601138 @default.
• W4307309199 cites W2113181569 @default.
• W4307309199 cites W2114042087 @default.
• W4307309199 cites W2123276271 @default.
• W4307309199 cites W2133465414 @default.
• W4307309199 cites W2158217645 @default.
• W4307309199 cites W2158878058 @default.
• W4307309199 cites W2463195069 @default.
• W4307309199 cites W2518341489 @default.
• W4307309199 cites W2550412681 @default.
• W4307309199 cites W2605795476 @default.
• W4307309199 cites W2607267860 @default.
• W4307309199 cites W2613539552 @default.
• W4307309199 cites W2750938144 @default.
• W4307309199 cites W2792807715 @default.
• W4307309199 cites W2897805491 @default.
• W4307309199 cites W2910650696 @default.
• W4307309199 cites W2945993165 @default.
• W4307309199 cites W2949802136 @default.
• W4307309199 cites W2985380082 @default.
• W4307309199 cites W2986758610 @default.
• W4307309199 cites W2998898616 @default.
• W4307309199 cites W3015473832 @default.
• W4307309199 cites W3045958371 @default.
• W4307309199 cites W3092426092 @default.
• W4307309199 cites W3124481539 @default.
• W4307309199 cites W3164630907 @default.
• W4307309199 cites W3188474217 @default.
• W4307309199 cites W4206498374 @default.
• W4307309199 cites W4223604229 @default.
• W4307309199 doi "https://doi.org/10.1371/journal.pone.0276285" @default.
• W4307309199 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36288343" @default.
• W4307309199 hasPublicationYear "2022" @default.
• W4307309199 type Work @default.
• W4307309199 citedByCount "2" @default.
• W4307309199 countsByYear W43073091992023 @default.
• W4307309199 crossrefType "journal-article" @default.
• W4307309199 hasAuthorship W4307309199A5002707544 @default.
• W4307309199 hasAuthorship W4307309199A5029387280 @default.
• W4307309199 hasAuthorship W4307309199A5034256236 @default.
• W4307309199 hasAuthorship W4307309199A5050697789 @default.
• W4307309199 hasAuthorship W4307309199A5055812269 @default.
• W4307309199 hasAuthorship W4307309199A5071892540 @default.
• W4307309199 hasAuthorship W4307309199A5073352935 @default.
• W4307309199 hasBestOaLocation W43073091991 @default.
• W4307309199 hasConcept C104317684 @default.
• W4307309199 hasConcept C126322002 @default.
• W4307309199 hasConcept C134018914 @default.
• W4307309199 hasConcept C167414201 @default.
• W4307309199 hasConcept C181199279 @default.
• W4307309199 hasConcept C185592680 @default.
• W4307309199 hasConcept C2778271984 @default.
• W4307309199 hasConcept C3018791406 @default.
• W4307309199 hasConcept C46111723 @default.
• W4307309199 hasConcept C55493867 @default.
• W4307309199 hasConcept C71829478 @default.
• W4307309199 hasConcept C71924100 @default.
• W4307309199 hasConcept C80311884 @default.
• W4307309199 hasConcept C86803240 @default.
• W4307309199 hasConcept C95444343 @default.
• W4307309199 hasConceptScore W4307309199C104317684 @default.
• W4307309199 hasConceptScore W4307309199C126322002 @default.
• W4307309199 hasConceptScore W4307309199C134018914 @default.
• W4307309199 hasConceptScore W4307309199C167414201 @default.
• W4307309199 hasConceptScore W4307309199C181199279 @default.
• W4307309199 hasConceptScore W4307309199C185592680 @default.
• W4307309199 hasConceptScore W4307309199C2778271984 @default.
• W4307309199 hasConceptScore W4307309199C3018791406 @default.
• W4307309199 hasConceptScore W4307309199C46111723 @default.
• W4307309199 hasConceptScore W4307309199C55493867 @default.
• W4307309199 hasConceptScore W4307309199C71829478 @default.
• W4307309199 hasConceptScore W4307309199C71924100 @default.
• W4307309199 hasConceptScore W4307309199C80311884 @default.
• W4307309199 hasConceptScore W4307309199C86803240 @default.
• W4307309199 hasConceptScore W4307309199C95444343 @default.
• W4307309199 hasIssue "10" @default.
• W4307309199 hasLocation W43073091991 @default.

• next