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• W4308120140 endingPage "13314" @default.
• W4308120140 startingPage "13314" @default.
• W4308120140 abstract "Nitrogen (N) is a major limiting factor for plant growth and crop production. The use of N fertilizer in forestry production is increasing each year, but the loss is substantial. Mastering the regulatory mechanisms of N uptake and transport is a key way to improve plant nitrogen use efficiency (NUE). However, this has rarely been studied in pecans. In this study, 10 AMT and 69 NRT gene family members were identified and systematically analyzed from the whole pecan genome using a bioinformatics approach, and the expression patterns of AMT and NRT genes and the uptake characteristics of NH4+ and NO3− in pecan were analyzed by aeroponic cultivation at varying NH4+/NO3− ratios (0/0, 0/100,25/75, 50/50, 75/25,100/0 as CK, T1, T2, T3, T4, and T5). The results showed that gene duplication was the main reason for the amplification of the AMT and NRT gene families in pecan, both of which experienced purifying selection. Based on qRT-PCR results, CiAMTs were primarily expressed in roots, and CiNRTs were majorly expressed in leaves, which were consistent with the distribution of pecan NH4+ and NO3− concentrations in the organs. The expression levels of CiAMTs and CiNRTs were mainly significantly upregulated under N deficiency and T4 treatment. Meanwhile, T4 treatment significantly increased the NH4+, NO3−, and NO2− concentrations as well as the Vmax and Km values of NH4+ and NO3− in pecans, and Vmax/Km indicated that pecan seedlings preferred to absorb NH4+. In summary, considering the single N source of T5, we suggested that the NH4+/NO3− ratio of 75:25 was more beneficial to improve the NUE of pecan, thus increasing pecan yield, which provides a theoretical basis for promoting the scale development of pecan and provides a basis for further identification of the functions of AMT and NRT genes in the N uptake and transport process of pecan." @default.
• W4308120140 created "2022-11-08" @default.
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• W4308120140 date "2022-11-01" @default.
• W4308120140 modified "2023-10-18" @default.
• W4308120140 title "Genome-Wide Identification and Expression Analysis of AMT and NRT Gene Family in Pecan (Carya illinoinensis) Seedlings Revealed a Preference for NH4+-N" @default.
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• W4308120140 doi "https://doi.org/10.3390/ijms232113314" @default.
• W4308120140 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36362101" @default.
• W4308120140 hasPublicationYear "2022" @default.
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