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• W4320492757 abstract "Surface plasmon resonance microscopy (SPRM) has been widely employed in biological fields because of its high spatial resolution and label-free detection modality. In this study, SPRM based on total internal reflection (TIR) is studied via a home-built SPRM system, and the principle of imaging of a single nanoparticle is analyzed as well. By designing a ring filter and combining it with the deconvolution algorithm in Fourier space, the parabolic tail of the nanoparticle image is removed, in which a spatial resolution of 248 nm is obtained. In addition, we also measured the specific binding between the human IgG antigen and goat anti-human IgG antibody using the TIR-based SPRM. The experimental results have proved that the system can image sparse nanoparticles and monitor biomolecular interactions." @default.
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• W4320492757 date "2023-02-12" @default.
• W4320492757 modified "2023-10-15" @default.
• W4320492757 title "Surface Plasmon Resonance Microscopy Based on Total Internal Reflection" @default.
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• W4320492757 doi "https://doi.org/10.3390/bios13020261" @default.
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