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• W4386333999 abstract "The rapid accumulation of single-cell RNA-seq data has provided rich resources to characterize various human cell populations. However, achieving accurate cell-type annotation using public references presents challenges due to inconsistent annotations, batch effects, and rare cell types. Here, we introduce SELINA (single-cell identity navigator), an integrative and automatic cell-type annotation framework based on a pre-curated reference atlas spanning various tissues. SELINA employs a multiple-adversarial domain adaptation network to remove batch effects within the reference dataset. Additionally, it enhances the annotation of less frequent cell types by synthetic minority oversampling and fits query data with the reference data using an autoencoder. SELINA culminates in the creation of a comprehensive and uniform reference atlas, encompassing 1.7 million cells covering 230 distinct human cell types. We substantiate its robustness and superiority across a multitude of human tissues. Notably, SELINA could accurately annotate cells within diverse disease contexts. SELINA provides a complete solution for human single-cell RNA-seq data annotation with both python and R packages." @default.
• W4386333999 created "2023-09-01" @default.
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• W4386333999 date "2023-09-01" @default.
• W4386333999 modified "2023-10-18" @default.
• W4386333999 title "Single-cell assignment using multiple-adversarial domain adaptation network with large-scale references" @default.
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• W4386333999 doi "https://doi.org/10.1016/j.crmeth.2023.100577" @default.
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