FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W965968077> ?p ?o ?g. }

• W965968077 endingPage "1838" @default.
• W965968077 startingPage "1830" @default.
• W965968077 abstract "We evaluated the efficacy of bioconjugation of oligodeoxynucleotides (ODNs) containing 1,4-dicarbonyl groups, a C4'-oxidized abasic site (OAS), and a newly designed 2'-methoxy analogue, via reductive amination with lysine residues. Dicarbonyls, aldehyde and ketone at C1- and C4-positions of deoxyribose in the ring-opened form of OAS allowed efficient reaction with amines. Kinetic studies indicated that reductive amination of OAS-containing ODNs with a proximal amine on the complementary strand proceeded 10 times faster than the corresponding reaction of an ODN containing an abasic site with C1-aldehyde. Efficient reductive amination between the DNA-binding domain of Escherichia coli DnaA protein and ODNs carrying OAS in the DnaA-binding sequence proceeded at the lysine residue in proximity to the phosphate group at the 5'-position of the OAS, in contrast to unsuccessful conjugation with abasic site ODNs, even though they have similar aldehydes. Theoretical calculation indicated that the C1-aldehyde of OAS was more accessible to the target lysine than that of the abasic site. These results demonstrate the potential utility of cross-linking strategies that use dicarbonyl-containing ODNs for the study of protein-nucleic acid interactions. Conjugation with a lysine-containing peptide that lacked specific affinity for ODN was also successful, further highlighting the advantages of 1,4-dicarbonyls." @default.
• W965968077 created "2016-06-24" @default.
• W965968077 creator A5025387150 @default.
• W965968077 creator A5049853860 @default.
• W965968077 creator A5055613862 @default.
• W965968077 creator A5057494257 @default.
• W965968077 creator A5063543498 @default.
• W965968077 creator A5067031114 @default.
• W965968077 creator A5074227066 @default.
• W965968077 date "2015-08-05" @default.
• W965968077 modified "2023-09-27" @default.
• W965968077 title "Bioconjugation of Oligodeoxynucleotides Carrying 1,4-Dicarbonyl Groups via Reductive Amination with Lysine Residues" @default.
• W965968077 cites W1967247681 @default.
• W965968077 cites W1968245541 @default.
• W965968077 cites W1971474873 @default.
• W965968077 cites W1982868413 @default.
• W965968077 cites W1983647569 @default.
• W965968077 cites W1985388948 @default.
• W965968077 cites W1991504955 @default.
• W965968077 cites W1992227890 @default.
• W965968077 cites W1993154064 @default.
• W965968077 cites W1993816098 @default.
• W965968077 cites W2001819226 @default.
• W965968077 cites W2002517416 @default.
• W965968077 cites W2006070503 @default.
• W965968077 cites W2011106168 @default.
• W965968077 cites W2038475484 @default.
• W965968077 cites W2039000608 @default.
• W965968077 cites W2039256798 @default.
• W965968077 cites W2041581133 @default.
• W965968077 cites W2042127800 @default.
• W965968077 cites W2045399258 @default.
• W965968077 cites W2050024759 @default.
• W965968077 cites W2052185232 @default.
• W965968077 cites W2053803024 @default.
• W965968077 cites W2071179774 @default.
• W965968077 cites W2074303404 @default.
• W965968077 cites W2077496082 @default.
• W965968077 cites W2081090776 @default.
• W965968077 cites W2090119175 @default.
• W965968077 cites W2106508579 @default.
• W965968077 cites W2110419228 @default.
• W965968077 cites W2112093431 @default.
• W965968077 cites W2112321284 @default.
• W965968077 cites W2128667181 @default.
• W965968077 cites W2147533449 @default.
• W965968077 cites W2157921498 @default.
• W965968077 cites W2173844423 @default.
• W965968077 cites W2330294318 @default.
• W965968077 cites W2333716116 @default.
• W965968077 cites W2036376784 @default.
• W965968077 doi "https://doi.org/10.1021/acs.bioconjchem.5b00361" @default.
• W965968077 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26200210" @default.
• W965968077 hasPublicationYear "2015" @default.
• W965968077 type Work @default.
• W965968077 sameAs 965968077 @default.
• W965968077 citedByCount "8" @default.
• W965968077 countsByYear W9659680772016 @default.
• W965968077 countsByYear W9659680772017 @default.
• W965968077 countsByYear W9659680772018 @default.
• W965968077 countsByYear W9659680772019 @default.
• W965968077 countsByYear W9659680772021 @default.
• W965968077 crossrefType "journal-article" @default.
• W965968077 hasAuthorship W965968077A5025387150 @default.
• W965968077 hasAuthorship W965968077A5049853860 @default.
• W965968077 hasAuthorship W965968077A5055613862 @default.
• W965968077 hasAuthorship W965968077A5057494257 @default.
• W965968077 hasAuthorship W965968077A5063543498 @default.
• W965968077 hasAuthorship W965968077A5067031114 @default.
• W965968077 hasAuthorship W965968077A5074227066 @default.
• W965968077 hasConcept C161790260 @default.
• W965968077 hasConcept C185592680 @default.
• W965968077 hasConcept C21951064 @default.
• W965968077 hasConcept C23963754 @default.
• W965968077 hasConcept C2776016237 @default.
• W965968077 hasConcept C2779825165 @default.
• W965968077 hasConcept C2779938440 @default.
• W965968077 hasConcept C2781338088 @default.
• W965968077 hasConcept C515207424 @default.
• W965968077 hasConcept C55493867 @default.
• W965968077 hasConcept C71240020 @default.
• W965968077 hasConceptScore W965968077C161790260 @default.
• W965968077 hasConceptScore W965968077C185592680 @default.
• W965968077 hasConceptScore W965968077C21951064 @default.
• W965968077 hasConceptScore W965968077C23963754 @default.
• W965968077 hasConceptScore W965968077C2776016237 @default.
• W965968077 hasConceptScore W965968077C2779825165 @default.
• W965968077 hasConceptScore W965968077C2779938440 @default.
• W965968077 hasConceptScore W965968077C2781338088 @default.
• W965968077 hasConceptScore W965968077C515207424 @default.
• W965968077 hasConceptScore W965968077C55493867 @default.
• W965968077 hasConceptScore W965968077C71240020 @default.
• W965968077 hasFunder F4320334764 @default.
• W965968077 hasIssue "8" @default.
• W965968077 hasLocation W9659680771 @default.
• W965968077 hasLocation W9659680772 @default.
• W965968077 hasOpenAccess W965968077 @default.
• W965968077 hasPrimaryLocation W9659680771 @default.

• next