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- FBcv_0005072 IAO_0000115 "The basic components of a polyA trap cassette are a promoter fused to a gene sequence that lacks a polyadenylation (polyA) sequence but is followed by a splice donor sequence. If the cassette is integrated into the genome outside a gene, the mRNA produced by the polyA trap is not polyadenylated and is expected to be rapidly degraded. However, if the cassette is integrated into an intron, the promoter in the polyA trap will drive expression of a transcriptional fusion containing the polyA gene trap fused to any downstream exons. The downstream terminal exon can provide a polyA signal, allowing stable expression of the gene encoded by the polyA trap. polyA trap cassettes can be used to report the expression of genes that are not normally expressed, or are expressed at very low levels, or may be used as a marker of successful integration of an insertion into a transcription unit. A polyA trap cassette may be inserted into a genome as part of a transgenic construct via transposable-element-mediated transgenesis, or may be inserted directly into a modified endogenous locus via a genome engineering method such as homologous recombination or CRISPR." @default.
- FBcv_0005072 contributor 0000-0002-0027-0858 @default.
- FBcv_0005072 date "2017-12-13T13:17:30.000Z" @default.
- FBcv_0005072 normalizedInformationContent "100" @default.
- FBcv_0005072 referenceCount "1" @default.
- FBcv_0005072 hasOBONamespace "experimental_tool_descriptor" @default.
- FBcv_0005072 id "FBcv:0005072" @default.
- FBcv_0005072 type Class @default.
- FBcv_0005072 isDefinedBy fbcv.owl @default.
- FBcv_0005072 label "polyA trap" @default.
- FBcv_0005072 subClassOf FBcv_0000000 @default.
- FBcv_0005072 subClassOf FBcv_0000013 @default.
- FBcv_0005072 subClassOf FBcv_0005001 @default.
- FBcv_0005072 subClassOf FBcv_0005068 @default.
- FBcv_0005072 subClassOf FBcv_0005072 @default.