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- FBcv_0007042 IAO_0000115 "An RMCE target element contains a region of DNA sequence flanked by a pair of target sites for a recombinase or an integrase, which are arranged such that this target sequence cassette can be replaced with donor sequence from a compatible RMCE donor element via recombination-mediated cassette exchange (RMCE). For RMCE mediated by a recombinase, the target cassette is flanked by two non-identical recombinase target sites that each carry a different mutation in the spacer sequence of the target site; this renders the two sites incompatible with each other. A compatible RMCE donor cassette is flanked by the same pair of non-identical sites; in the presence of recombinase, a double recombination event between the identical sites on the donor and target sequences results in replacement of the target cassette with the donor cassette. In the case of RMCE mediated by an integrase, the RMCE cassette is flanked by identical integrase target sites, while a compatible RMCE donor cassette is flanked by copies of the orthogonal integrase target site; in the presence of integrase, a double recombination event results in the target cassette being replaced with that of the donor cassette. In this case, if the flanking sites are in inverted orientation, replacement of the target cassette can occur in either direction." @default.
- FBcv_0007042 contributor 0000-0002-1373-1705 @default.
- FBcv_0007042 date "2020-02-03T14:52:50.000Z" @default.
- FBcv_0007042 normalizedInformationContent "100" @default.
- FBcv_0007042 referenceCount "1" @default.
- FBcv_0007042 hasExactSynonym "recombination-mediated cassette exchange target element" @default.
- FBcv_0007042 hasOBONamespace "experimental_tool_descriptor" @default.
- FBcv_0007042 id "FBcv:0007042" @default.
- FBcv_0007042 type Class @default.
- FBcv_0007042 isDefinedBy fbcv.owl @default.
- FBcv_0007042 label "RMCE target element" @default.
- FBcv_0007042 subClassOf FBcv_0000000 @default.
- FBcv_0007042 subClassOf FBcv_0000013 @default.
- FBcv_0007042 subClassOf FBcv_0005001 @default.
- FBcv_0007042 subClassOf FBcv_0005075 @default.
- FBcv_0007042 subClassOf FBcv_0007042 @default.