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- MI_0009 IAO_0000115 "The protein of interest is presented on the outer membrane of Gram negative bacteria by expressing it as a fusion partner to peptide signals that direct heterologous proteins to the cell surface. For instance, a single chain Fv (scFv) antibody fragment, consisting of the variable heavy and variable light domains from two separate anti-digoxin monoclonal antibodies, was displayed on the outer membrane of Escherichia coli by fusing it to an Lpp-OmpA. Similar systems have also been developed for gram positive bacteria. Fluorescence-activated cell sorting (FACS), is used to specifically select clones displaying a protein binding to scFv-producing cells." @default.
- MI_0009 normalizedInformationContent "100" @default.
- MI_0009 referenceCount "1" @default.
- MI_0009 hasOBONamespace "PSI-MI" @default.
- MI_0009 id "MI:0009" @default.
- MI_0009 inSubset PSI-MI_slim @default.
- MI_0009 type Class @default.
- MI_0009 isDefinedBy mi.owl @default.
- MI_0009 label "bacterial display" @default.
- MI_0009 subClassOf MI_0000 @default.
- MI_0009 subClassOf MI_0001 @default.
- MI_0009 subClassOf MI_0009 @default.
- MI_0009 subClassOf MI_0013 @default.
- MI_0009 subClassOf MI_0034 @default.
- MI_0009 subClassOf MI_0045 @default.
- MI_0009 subClassOf MI_0051 @default.
- MI_0009 subClassOf MI_0054 @default.
- MI_0009 subClassOf MI_0400 @default.
- MI_0009 subClassOf MI_0401 @default.