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- MI_0014 IAO_0000115 "Adenylate cyclase is encoded by the cyaA gene and contains a catalytic domain which can be proteolytically cleaved into two complementary fragments, T25 and T18, which remain associated in the presence of calmodulin in a fully active ternary complex. In the absence of calmodulin, the mixture of the two fragments does not exhibit detectable activity, suggesting that the two fragments do not associate. When expressed in an adenylate cyclase-deficient E. coli strain (E. coli lacks calmodulin or calmodulin-related proteins), the T25 and T18 fragments fused to putative interacting proteins are brought into close association which result in cAMP synthesis. The level of reconstructed adenylate cyclase can be estimated by monitoring the expression of a cAMP dependent reporter gene. The T25 tagged protein is generally regarded as the bait, the T18 as the prey." @default.
- MI_0014 normalizedInformationContent "100" @default.
- MI_0014 referenceCount "1" @default.
- MI_0014 hasExactSynonym "adenylate cyclase" @default.
- MI_0014 hasExactSynonym "bacterial two-hybrid" @default.
- MI_0014 hasOBONamespace "PSI-MI" @default.
- MI_0014 id "MI:0014" @default.
- MI_0014 inSubset PSI-MI_slim @default.
- MI_0014 type Class @default.
- MI_0014 isDefinedBy mi.owl @default.
- MI_0014 label "adenylate cyclase complementation" @default.
- MI_0014 subClassOf MI_0000 @default.
- MI_0014 subClassOf MI_0001 @default.
- MI_0014 subClassOf MI_0014 @default.
- MI_0014 subClassOf MI_0045 @default.
- MI_0014 subClassOf MI_0090 @default.