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- MI_0018 IAO_0000115 "The classical two-hybrid system is a method that uses transcriptional activity as a measure of protein-protein interaction. It relies on the modular nature of many site-specific transcriptional activators (GAL 4) , which consist of a DNA-binding domain and a transcriptional activation domain. The DNA-binding domain serves to target the activator to the specific genes that will be expressed, and the activation domain contacts other proteins of the transcriptional machinery to enable transcription to occur. The two-hybrid system is based on the observation that the two domains of the activator need to be non-covalently brought together by the interaction of any two proteins. The application of this system requires the expression of two hybrid. Generally this assay is performed in yeast cell, but it can also be carried out in other organism. The bait protein is fused to the DNA binding molecule, the prey to the transcriptional activator." @default.
- MI_0018 normalizedInformationContent "82.536929774922015" @default.
- MI_0018 referenceCount "14" @default.
- MI_0018 hasExactSynonym "2 hybrid" @default.
- MI_0018 hasExactSynonym "2-hybrid" @default.
- MI_0018 hasExactSynonym "2H" @default.
- MI_0018 hasExactSynonym "2h" @default.
- MI_0018 hasExactSynonym "Gal4 transcription regeneration" @default.
- MI_0018 hasExactSynonym "Y2H" @default.
- MI_0018 hasExactSynonym "classical two hybrid" @default.
- MI_0018 hasExactSynonym "two-hybrid" @default.
- MI_0018 hasExactSynonym "yeast two hybrid" @default.
- MI_0018 hasOBONamespace "PSI-MI" @default.
- MI_0018 hasRelatedSynonym "Y-2H" @default.
- MI_0018 id "MI:0018" @default.
- MI_0018 inSubset PSI-MI_slim @default.
- MI_0018 type Class @default.
- MI_0018 isDefinedBy mi.owl @default.
- MI_0018 label "two hybrid" @default.
- MI_0018 subClassOf MI_0000 @default.
- MI_0018 subClassOf MI_0001 @default.
- MI_0018 subClassOf MI_0018 @default.
- MI_0018 subClassOf MI_0045 @default.
- MI_0018 subClassOf MI_0090 @default.
- MI_0018 subClassOf MI_0232 @default.