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- MI_0021 IAO_0000115 "Two proteins can be localised to cell compartments, in the same experiment, if they are expressed as chimeric proteins fused to distinct proteins fluorescing at different wavelengths (Green Fluorescent Protein and Red Fluorescent Protein for example). Using a confocal microscope the two proteins can be visualized in living cells and it can be determined whether they have the same subcellular location. Fluorescence microscopy of cells expressing a GFP fusion protein can also demonstrate dynamic processes such as its translocation from one subcellular compartment to another. OBSOLETE: use imaging technique (MI:0428) and specific probe as feature of each interacting protein." @default.
- MI_0021 normalizedInformationContent "100" @default.
- MI_0021 referenceCount "1" @default.
- MI_0021 hasExactSynonym "coloc fluoresc probe" @default.
- MI_0021 hasOBONamespace "PSI-MI" @default.
- MI_0021 id "MI:0021" @default.
- MI_0021 inSubset PSI-MI_slim @default.
- MI_0021 type Class @default.
- MI_0021 isDefinedBy mi.owl @default.
- MI_0021 label "colocalization by fluorescent probes cloning" @default.
- MI_0021 subClassOf MI_0021 @default.
- MI_0021 subClassOf Thing @default.
- MI_0021 deprecated "true" @default.