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- MI_0022 IAO_0000115 "The subcellular location of a protein can be demonstrated by treating cells fixed on a microscope slide with an antibody specific for the protein of interest. A secondary antibody conjugated with a reactive enzyme (e.g. horseradish peroxidase) is then added. Following a washing step to remove the unbound secondary ligand, a chromogenic substrate (e.g. 3,3', 5,5' tetramethyl benzidine chromogen [TMB]) is converted to a soluble coloured product by the conjugated enzyme and can then be visualised by standard microscopic techniques. OBSOLETE since combination of Interaction Detection Method and Interaction Type.Consider using the Interaction Detection Method imaging techniques (MI:0428) coupled with Interaction Type colocalisation (MI:0403) and Participant detection immunostaining (MI:0422) instead." @default.
- MI_0022 normalizedInformationContent "100" @default.
- MI_0022 referenceCount "1" @default.
- MI_0022 hasExactSynonym "Immunofluorescence Staining" @default.
- MI_0022 hasExactSynonym "Immunostaining" @default.
- MI_0022 hasExactSynonym "coloc immunostaining" @default.
- MI_0022 hasOBONamespace "PSI-MI" @default.
- MI_0022 id "MI:0022" @default.
- MI_0022 inSubset PSI-MI_slim @default.
- MI_0022 type Class @default.
- MI_0022 isDefinedBy mi.owl @default.
- MI_0022 label "colocalization by immunostaining" @default.
- MI_0022 subClassOf MI_0022 @default.
- MI_0022 subClassOf Thing @default.
- MI_0022 deprecated "true" @default.