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- MI_0031 IAO_0000115 "Cross-linking agents induce the formation of covalent bonds among proteins that are neighbours. The cross-linker may be a bifunctional molecule having two reactive ends linked by a spacer, often containing a disulfide bond. When a reducing agent is added the disulfide bridge is cleaved, the cross-linked pairs are released and can be identified. There are various classes of cross-linkers, the most common are those having photoreactive groups that become reactive fluorophores when activated by UV light thereby resulting in photolabeling the cross-linked moieties." @default.
- MI_0031 normalizedInformationContent "100" @default.
- MI_0031 referenceCount "1" @default.
- MI_0031 hasExactSynonym "Label transfer techniques" @default.
- MI_0031 hasExactSynonym "Photoaffinity labelling" @default.
- MI_0031 hasExactSynonym "bifunctional agent crosslink" @default.
- MI_0031 hasOBONamespace "PSI-MI" @default.
- MI_0031 id "MI:0031" @default.
- MI_0031 inSubset PSI-MI_slim @default.
- MI_0031 type Class @default.
- MI_0031 isDefinedBy mi.owl @default.
- MI_0031 label "protein cross-linking with a bifunctional reagent" @default.
- MI_0031 subClassOf MI_0000 @default.
- MI_0031 subClassOf MI_0001 @default.
- MI_0031 subClassOf MI_0030 @default.
- MI_0031 subClassOf MI_0031 @default.
- MI_0031 subClassOf MI_0045 @default.
- MI_0031 subClassOf MI_0401 @default.