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- MI_0032 IAO_0000115 "The strategy to determine the complete amino acid sequence of a protein by mass spectrometry relies on the generation of a nested set of fragments differing by one amino acid. This reveals the identity of the residue that has been removed at each degradation step by measuring the mass difference of fragments differing of one residue. Peptide fragments can be obtained by protease treatment combined with the fragmentation promoted by collision (or other methods) within a tandem mass spectrometer. This approach can be carried out with LC MS/MS (Liquid Chromatography Tandem Mass Spectrometry), nanoESI MS/MS (nanoElectrospray Ionisation tandem mass spectrometry), or FTMS (Fourier Transform mass spectrometry) instruments." @default.
- MI_0032 normalizedInformationContent "100" @default.
- MI_0032 referenceCount "1" @default.
- MI_0032 hasExactSynonym "de novo protein sequence" @default.
- MI_0032 hasOBONamespace "PSI-MI" @default.
- MI_0032 hasRelatedSynonym "MS/MS" @default.
- MI_0032 id "MI:0032" @default.
- MI_0032 inSubset PSI-MI_slim @default.
- MI_0032 type Class @default.
- MI_0032 isDefinedBy mi.owl @default.
- MI_0032 label "de novo protein sequencing by mass spectrometry" @default.
- MI_0032 subClassOf MI_0000 @default.
- MI_0032 subClassOf MI_0002 @default.
- MI_0032 subClassOf MI_0003 @default.
- MI_0032 subClassOf MI_0032 @default.
- MI_0032 subClassOf MI_0093 @default.
- MI_0032 subClassOf MI_0427 @default.
- MI_0032 subClassOf MI_0659 @default.
- MI_0032 subClassOf MI_0661 @default.