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- MI_0049 IAO_0000115 "A method in which separation depends upon the ability of one participant to bind to a filter or membrane which the other participants do not. Molecules interacting with the bound molecule will also be retain on the filter. For example, proteins expressed by different clones of an expression library are bound to a nitrocellulose membrane, by colony (bacterial library) or plaque (phage library) blotting. A labelled protein can then be used as a probe to identify clones expressing proteins that interact with the probe. Interactions occur on the nitrocellulose filters. The method is highly general and therefore widely applicable. A variety of approaches can be used to label the ligand, alternatively the ligand can be detected by a specific antibody." @default.
- MI_0049 normalizedInformationContent "100" @default.
- MI_0049 referenceCount "1" @default.
- MI_0049 hasExactSynonym "Filter overlay assay" @default.
- MI_0049 hasOBONamespace "PSI-MI" @default.
- MI_0049 hasRelatedSynonym "dot blot" @default.
- MI_0049 id "MI:0049" @default.
- MI_0049 inSubset PSI-MI_slim @default.
- MI_0049 type Class @default.
- MI_0049 isDefinedBy mi.owl @default.
- MI_0049 label "filter binding" @default.
- MI_0049 subClassOf MI_0000 @default.
- MI_0049 subClassOf MI_0001 @default.
- MI_0049 subClassOf MI_0045 @default.
- MI_0049 subClassOf MI_0049 @default.
- MI_0049 subClassOf MI_0400 @default.
- MI_0049 subClassOf MI_0401 @default.
- MI_0049 subClassOf MI_0892 @default.