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- MI_0052 IAO_0000115 "FCS monitors the random motion of fluorescently labelled molecules inside a defined volume irradiated by a focused laser beam. These fluctuations provide information on the rate of diffusion or diffusion time of a particle and this is directly dependent on the particle mass. As a consequence, any increase in the mass of a biomolecule, e.g. as a result of an interaction with a second molecule, is readily detected as an increase in the diffusion time of the particle. From these results the concentration of the different molecules can be calculated as well as their binding constant." @default.
- MI_0052 normalizedInformationContent "100" @default.
- MI_0052 referenceCount "1" @default.
- MI_0052 hasExactSynonym "FCS" @default.
- MI_0052 hasExactSynonym "fcs" @default.
- MI_0052 hasOBONamespace "PSI-MI" @default.
- MI_0052 hasRelatedSynonym "fluctuation correlation specctrometry" @default.
- MI_0052 id "MI:0052" @default.
- MI_0052 inSubset PSI-MI_slim @default.
- MI_0052 type Class @default.
- MI_0052 isDefinedBy mi.owl @default.
- MI_0052 label "fluorescence correlation spectroscopy" @default.
- MI_0052 subClassOf MI_0000 @default.
- MI_0052 subClassOf MI_0001 @default.
- MI_0052 subClassOf MI_0013 @default.
- MI_0052 subClassOf MI_0045 @default.
- MI_0052 subClassOf MI_0051 @default.
- MI_0052 subClassOf MI_0052 @default.