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- MI_0055 IAO_0000115 "FRET is a quantum mechanical process involving the radiationless transfer of energy from a donor fluorophore to an appropriately positioned acceptor fluorophore. The fluorophores are genetically fused to the protein in analysis and cotransfected. Three basic conditions must be fulfilled for FRET to occur between a donor molecule and acceptor molecule. First, the donor emission spectrum must significantly overlap the absorption spectrum of the acceptor. Second, the distance between the donor and acceptor fluorophores must fall within the range 20 to 100 Angstrom. Third, the donor and acceptor fluorophores must be in favourable orientations." @default.
- MI_0055 normalizedInformationContent "100" @default.
- MI_0055 referenceCount "1" @default.
- MI_0055 hasExactSynonym "FRET analysis" @default.
- MI_0055 hasExactSynonym "FRET" @default.
- MI_0055 hasExactSynonym "RET" @default.
- MI_0055 hasExactSynonym "fret" @default.
- MI_0055 hasOBONamespace "PSI-MI" @default.
- MI_0055 id "MI:0055" @default.
- MI_0055 inSubset PSI-MI_slim @default.
- MI_0055 type Class @default.
- MI_0055 isDefinedBy mi.owl @default.
- MI_0055 label "fluorescent resonance energy transfer" @default.
- MI_0055 subClassOf MI_0000 @default.
- MI_0055 subClassOf MI_0001 @default.
- MI_0055 subClassOf MI_0013 @default.
- MI_0055 subClassOf MI_0045 @default.
- MI_0055 subClassOf MI_0051 @default.
- MI_0055 subClassOf MI_0055 @default.