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- MI_0071 IAO_0000115 "In sizing columns (gel filtration), the elution position of a protein or of a complex depends on its Stokes radius. Molecules with a radius that is smaller than the bead size are retained and retarded by the interaction with the matrix. The observation that two proteins, loaded on a sieving column, elute in a fraction(s) corresponding to a MW that is larger than the MW of either protein may be taken as an indication that the two proteins interact. Furthermore this technique provides a conceptually simple method for evaluating the affinity of the interaction." @default.
- MI_0071 normalizedInformationContent "100" @default.
- MI_0071 referenceCount "1" @default.
- MI_0071 hasExactSynonym "Gel Filtration" @default.
- MI_0071 hasExactSynonym "Size Exclusion Chromatography" @default.
- MI_0071 hasExactSynonym "Sizing column" @default.
- MI_0071 hasOBONamespace "PSI-MI" @default.
- MI_0071 id "MI:0071" @default.
- MI_0071 inSubset PSI-MI_slim @default.
- MI_0071 type Class @default.
- MI_0071 isDefinedBy mi.owl @default.
- MI_0071 label "molecular sieving" @default.
- MI_0071 subClassOf MI_0000 @default.
- MI_0071 subClassOf MI_0001 @default.
- MI_0071 subClassOf MI_0013 @default.
- MI_0071 subClassOf MI_0045 @default.
- MI_0071 subClassOf MI_0071 @default.
- MI_0071 subClassOf MI_0091 @default.
- MI_0071 subClassOf MI_0401 @default.