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- MI_0082 IAO_0000115 "This approach leads to protein identification by matching peptide masses, as measured by mass spectrometry, to the ones calculated from in silico fragmentation of a protein sequence database. A peptide mixture from a tryptic digest is analysed by MALDI-MS (Matrix-assisted laser desorption ionization mass spectrometry). The list of peptide masses obtained by MALDI MS is automatically compared to the calculated masses of the predicted peptide fragments for each entry in the database. High mass accuracy is very important in order to obtain a statistically significant and unambiguous match This method is best applied to completely sequenced genomes and well characterised proteomes. However, depending on the data quality, proteins that are highly homologous to already characterised proteins (greater than 80 to 90% sequence identity) can also be identified. The retrieved sequence are evaluated by mass accuracy of the peptides, matching of additional peptide masses in the MALDI spectrum after accounting for common modifications such as oxidation, acrylamidation of cysteine and missed cleavages and the use of secondary information (apparent isoelectric point and molecular weight). If any ambiguity about the identification by MALDI-MS still exists, the results must verified by an other identification method. Peptide mass fingerprint is generally carried out with a MALDI-TOF (Matrix-assisted laser desorption ionization time-of-flight ) instrument but can also be achieved ESI-TOF (Electrospray Ionisation time-of-flight) or LC-MS (Liquid Chromatography-Mass Spectrometry) mass spectrometer." @default.
- MI_0082 normalizedInformationContent "100" @default.
- MI_0082 referenceCount "1" @default.
- MI_0082 hasExactSynonym "fingerprinting" @default.
- MI_0082 hasOBONamespace "PSI-MI" @default.
- MI_0082 id "MI:0082" @default.
- MI_0082 inSubset PSI-MI_slim @default.
- MI_0082 type Class @default.
- MI_0082 isDefinedBy mi.owl @default.
- MI_0082 label "peptide massfingerprinting" @default.
- MI_0082 subClassOf MI_0000 @default.
- MI_0082 subClassOf MI_0002 @default.
- MI_0082 subClassOf MI_0003 @default.
- MI_0082 subClassOf MI_0082 @default.
- MI_0082 subClassOf MI_0093 @default.
- MI_0082 subClassOf MI_0427 @default.
- MI_0082 subClassOf MI_0433 @default.
- MI_0082 subClassOf MI_0659 @default.
- MI_0082 subClassOf MI_0661 @default.