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- MI_0090 IAO_0000115 "In the protein-fragment complementation assay, the proteins of interest ("Bait" and "Prey") are covalently linked at the genetic level to incomplete fragments of a third protein (known as the "reporter") and are expressed in vivo, Interaction between the "bait" and the "prey" proteins brings the fragments of the "reporter" protein in close enough proximity to allow them to reform and become the functional reporter protein. Typically enzymes which confer resistance to antibiotics, such as Dihydrofolate reductase or Beta-lactamase, or proteins that give colorimetric or fluorescent signals are used. The Bait protein is generally the protein under study and the methods are readily adaptable to highthroughput mode." @default.
- MI_0090 normalizedInformationContent "75.42669154663659" @default.
- MI_0090 referenceCount "41" @default.
- MI_0090 hasExactSynonym "PCA" @default.
- MI_0090 hasExactSynonym "complementation" @default.
- MI_0090 hasOBONamespace "PSI-MI" @default.
- MI_0090 id "MI:0090" @default.
- MI_0090 inSubset PSI-MI_slim @default.
- MI_0090 type Class @default.
- MI_0090 isDefinedBy mi.owl @default.
- MI_0090 label "protein complementation assay" @default.
- MI_0090 subClassOf MI_0000 @default.
- MI_0090 subClassOf MI_0001 @default.
- MI_0090 subClassOf MI_0045 @default.
- MI_0090 subClassOf MI_0090 @default.