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- MI_0094 IAO_0000115 "A wide range of dyes have been used over the years to visualise proteins in polyacrylamide gels - Coomasie Blue and silver-staining being two classical methods. Fluorescent dyes such as Nile Red and SYPRO Orange are now increasingly used due to their superior dynamic range. Use of non-denaturing gels can allow visualisation of protein protein interactions. Several dyes can be used to specifically indicate residue modification, however this methodology will give no information as the number of residues modified or their position within the protein sequence. Examples include the use of acid fuscian or the fluorescent dansyl hydrazine to show protein glycosylation." @default.
- MI_0094 normalizedInformationContent "100" @default.
- MI_0094 referenceCount "1" @default.
- MI_0094 hasOBONamespace "PSI-MI" @default.
- MI_0094 id "MI:0094" @default.
- MI_0094 inSubset PSI-MI_slim @default.
- MI_0094 type Class @default.
- MI_0094 isDefinedBy mi.owl @default.
- MI_0094 label "protein staining" @default.
- MI_0094 subClassOf MI_0000 @default.
- MI_0094 subClassOf MI_0003 @default.
- MI_0094 subClassOf MI_0094 @default.
- MI_0094 subClassOf MI_0659 @default.