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- MI_0095 IAO_0000115 "ProteinChip(r) Array technology is a surface-enhanced laser desorption/ionization (SELDI) approach (Ciphergen Biosystems Inc. Fremont, CA, USA) for sample fractionation accomplished by retentate chromatography. Retentate chromatography is performed on ProteinChip Arrays with varying chromatographic properties (e.g. anion exchange, cation exchange, metal affinity and reverse phase). By utilising arrays with differing surface chemistries in parallel and in series, a complex mixture of proteins, as from cells or body fluids, can be resolved into subsets of proteins with common properties. Specific analytes can also be examined by using preactivated arrays to which a bait molecule (such as an antibody or biotinylated DNA) is immobilized and a solution containing the binding partner(s) is presented to the array. This array-based immunoprecipitation or protein-binding experiment has been used with good success to study DNA-binding proteins, receptor-ligand interactions, and protein complexes. Any ligand retained on a SELDI chip can directly be identified by mass spectrometry." @default.
- MI_0095 normalizedInformationContent "100" @default.
- MI_0095 referenceCount "1" @default.
- MI_0095 hasExactSynonym "SELDI ProteinChip" @default.
- MI_0095 hasExactSynonym "seldi chip" @default.
- MI_0095 hasOBONamespace "PSI-MI" @default.
- MI_0095 id "MI:0095" @default.
- MI_0095 inSubset PSI-MI_slim @default.
- MI_0095 type Class @default.
- MI_0095 isDefinedBy mi.owl @default.
- MI_0095 label "proteinchip(r) on a surface-enhanced laser desorption/ionization" @default.
- MI_0095 subClassOf MI_0000 @default.
- MI_0095 subClassOf MI_0001 @default.
- MI_0095 subClassOf MI_0008 @default.
- MI_0095 subClassOf MI_0045 @default.
- MI_0095 subClassOf MI_0089 @default.
- MI_0095 subClassOf MI_0095 @default.
- MI_0095 subClassOf MI_0400 @default.
- MI_0095 subClassOf MI_0401 @default.