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- MI_0097 IAO_0000115 "In this complementation approach the bait can be any membrane protein (for example a receptor or a channel protein), the prey is cloned as a fusion protein of any cDNA from a library and the coding sequence of cytoplasmic RAS (cdc25 in yeast). If the bait and the prey interact, RAS is recruited close to the membrane and can activate cell growth. This procedure must take place in cells having a mutated RAS (Cdc25-2 yeast strain having a temperature sensitive mutation of RAS) to avoid constitutive growth activation." @default.
- MI_0097 normalizedInformationContent "100" @default.
- MI_0097 referenceCount "1" @default.
- MI_0097 hasExactSynonym "reverse RRS" @default.
- MI_0097 hasExactSynonym "reverse rrs" @default.
- MI_0097 hasOBONamespace "PSI-MI" @default.
- MI_0097 id "MI:0097" @default.
- MI_0097 inSubset PSI-MI_slim @default.
- MI_0097 type Class @default.
- MI_0097 isDefinedBy mi.owl @default.
- MI_0097 label "reverse ras recruitment system" @default.
- MI_0097 subClassOf MI_0000 @default.
- MI_0097 subClassOf MI_0001 @default.
- MI_0097 subClassOf MI_0045 @default.
- MI_0097 subClassOf MI_0090 @default.
- MI_0097 subClassOf MI_0097 @default.