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- MI_0098 IAO_0000115 "This method permits the coupling of phenotype to genotype via the formation of a non-covalent ternary complex between mRNAs and their encoded polypeptides while they are translated in an in vitro system. As a first step a cDNA library is constructed that encodes chimeric proteins in which the natural proteins or protein domains are fused to a C-terminal tether. As a consequence when the mRNA is translated in vitro the domain can fold while the tether is still in the ribosomal tunnel. Furthermore this chimeric mRNAs lack a stop codon, thus preventing release of the mRNA and the polypeptide from the ribosome. High concentrations of magnesium and low temperature further stabilise the ternary complex. Similarly to phage display, these complexes can be used directly to select for nucleic acids encoding proteins with desired properties." @default.
- MI_0098 normalizedInformationContent "100" @default.
- MI_0098 referenceCount "1" @default.
- MI_0098 hasOBONamespace "PSI-MI" @default.
- MI_0098 id "MI:0098" @default.
- MI_0098 inSubset PSI-MI_slim @default.
- MI_0098 type Class @default.
- MI_0098 isDefinedBy mi.owl @default.
- MI_0098 label "ribosome display" @default.
- MI_0098 subClassOf MI_0000 @default.
- MI_0098 subClassOf MI_0001 @default.
- MI_0098 subClassOf MI_0034 @default.
- MI_0098 subClassOf MI_0045 @default.
- MI_0098 subClassOf MI_0098 @default.
- MI_0098 subClassOf MI_0400 @default.
- MI_0098 subClassOf MI_0401 @default.