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- MI_0099 IAO_0000115 "SPA relies upon the fact that a beta particle emitted from a radioisotope decay can excite a fluorophore only when it is at a very short distance in water solution (few micrometers). The ligand is labelled with a radioactive atom and its potential partner is fixed to fluorophore containing beads, the emitted fluorescence proving their interaction can be measured in a scintillation counter. The scintillator measures only the amount of bound radiolabelled ligand. Competition experiment with cold competitor can be done to estimate the binding affinities (50% inhibitory concentration [IC50], cold ligand versus labelled ligand). Loss of signal can also be used to measure substrate cleavage by an enzyme, and labelled antibodies used to titrate the degree of modified residue present." @default.
- MI_0099 normalizedInformationContent "95.413332725061409" @default.
- MI_0099 referenceCount "2" @default.
- MI_0099 hasExactSynonym "RIA Radio Immuno Assay" @default.
- MI_0099 hasExactSynonym "SPA" @default.
- MI_0099 hasExactSynonym "spa" @default.
- MI_0099 hasOBONamespace "PSI-MI" @default.
- MI_0099 id "MI:0099" @default.
- MI_0099 inSubset PSI-MI_slim @default.
- MI_0099 type Class @default.
- MI_0099 isDefinedBy mi.owl @default.
- MI_0099 label "scintillation proximity assay" @default.
- MI_0099 subClassOf MI_0000 @default.
- MI_0099 subClassOf MI_0001 @default.
- MI_0099 subClassOf MI_0013 @default.
- MI_0099 subClassOf MI_0045 @default.
- MI_0099 subClassOf MI_0099 @default.